Iso-seco-tanapartholide activates Nrf2 signaling pathway through Keap1 modification and oligomerization to exert anti-inflammatory effects

• Published in: Free radical biology & medicine Vol. 178; pp. 398 - 412
• Main Authors: Zhu, Dongrong, Xia, Yuanzheng, Li, Shang, Kong, Min, Chen, Chen, Xue, Guimin, Kong, Lingyi, Luo, Jianguang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2022
• Subjects: Anti-inflammatory; Anti-Inflammatory Agents - pharmacology; Iso-seco-tanapartholide; Keap1 dimer; Kelch-Like ECH-Associated Protein 1 - genetics; Kelch-Like ECH-Associated Protein 1 - metabolism; NF-E2-Related Factor 2 - genetics; NF-E2-Related Factor 2 - metabolism; NF-kappa B - genetics; NF-kappa B - metabolism; Nrf2; Oligomerization; Signal Transduction; NO; Oligomerization; DTT; H&E; βME; DARTS; IST; LPS; CETSA; NAC; RNA-seq; ARE; Nrf2; DEX; Keap1 dimer; ROS; Iso-seco-tanapartholide; Keap1; Anti-inflammatory; GSH; ELISA

Covalent modification of Keap1 results in reducing ubiquitination and the accumulation of Nrf2, which subsequently initiates the transcription of cellular anti-oxidant and anti-inflammatory genes. Iso-seco-tanapartholide (IST), a sesquiterpene isolated from the traditional Chinese medicine Artemisia...