In vivo Protein Evolution, Next Generation Protein Engineering Strategy: from Random Approach to Target-specific Approach

• Published in: Biotechnology and bioprocess engineering Vol. 24; no. 1; pp. 85 - 94
• Main Authors: Kim, Jin Young, Yoo, Hee-Wang, Lee, Pyung-Gang, Lee, Sun-Gu, Seo, Joo-Hyun, Kim, Byung-Gee
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Biotechnology and Bioengineering 01.02.2019; Springer Nature B.V; 한국생물공학회
• Subjects: Biotechnology; Chemistry; Chemistry and Materials Science; DNA; Evolution; In vitro methods and tests; In vivo methods and tests; Industrial and Production Engineering; Libraries; Mutagenesis; mutagens; Mutants; prices; Protein engineering; Proteins; Random mutagenesis; Review Paper; 생물공학; random; focused library; protein evolution; screening method; target-specific

In vivo protein evolution is a protein engineering approach that is performed by both generating mutagenesis libraries and selecting desired mutants in a cell. Despite its clear advantages in some aspects, the approach has not much been popularized compared to in vitro protein evolution methods whic...