Lyase activity of glycogen synthase: Is an elimination/addition mechanism a possible reaction pathway for retaining glycosyltransferases?

• Published in: IUBMB life Vol. 64; no. 7; p. 649
• Main Authors: Díaz, Adelaida, Díaz-Lobo, Mireia, Grados, Enrique, Guinovart, Joan J, Fita, Ignacio, Ferrer, Joan C
• Format: Journal Article
• Language: English
• Published: England 01.07.2012
• Subjects: Binding Sites; Biochemistry - methods; Catalysis; Cations; Chromatography, Liquid - methods; DNA, Complementary - metabolism; Gas Chromatography-Mass Spectrometry - methods; Glycogen Synthase - chemistry; Glycogen Synthase - metabolism; Glycosyltransferases - chemistry; Lyases - chemistry; Models, Chemical; Molecular Conformation; Oxygen - chemistry; Protons; Uridine Diphosphate - chemistry; X-Ray Diffraction

Despite the biological relevance of glycosyltrasferases (GTs) and the many efforts devoted to this subject, the catalytic mechanism through which a subclass of this large family of enzymes, namely those that operate with net retention of the anomeric configuration, has not been fully established. He...