Sixteen novel microsatellite markers developed for Dendrocalamus sinicus (Poaceae), the strongest woody bamboo in the world

• Premise of the study: Microsatellite primers were developed in the strongest and most economically important bamboo species, Dendrocalamus sinicus, to investigate its genetic variability.• Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) protocol, 16 p...

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Published inAmerican journal of botany Vol. 99; no. 9; pp. e347 - e349
Main Authors Dong, Yu-Ran, Zhang, Zhi-Rong, Yang, Han-Qi
Format Journal Article
LanguageEnglish
Published United States Botanical Society of America 01.09.2012
Botanical Society of America, Inc
Subjects
alleles
amplified fragment length polymorphism
Bamboo
bamboos
Bambusa - genetics
Botany
China
Conservation
Dendrocalamus
Dendrocalamus sinicus
DNA primers
DNA Primers - metabolism
Genetic diversity
genetic variation
loci
microsatellite markers
microsatellite repeats
Microsatellite Repeats - genetics
Molecular Sequence Data
Poaceae
Polymorphism, Genetic
Species Specificity
Wood
Wood - genetics
China
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Summary:• Premise of the study: Microsatellite primers were developed in the strongest and most economically important bamboo species, Dendrocalamus sinicus, to investigate its genetic variability.• Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) protocol, 16 polymorphic primer sets were identified within 24 individuals from two geographic locations. The number of alleles per locus ranged from two to four, with a mean of 2.6. The observed and expected heterozygosities varied from 0 to 1.000 and from 0.311 to 0.754, respectively. The cross-amplification of the 16 primer pairs was tested in four other Dendrocalamus species, and nine to 13 (56.3–81.3%) primer pairs were successfully amplified.• Conclusions: These markers will be useful for studies on the genetic diversity of D. sinicus, which may facilitate conservation of this species.
Bibliography:http://dx.doi.org/10.3732/ajb.1200029
These authors contributed equally to this work.
The authors thank the reviewing editor for valuable suggestions and comments on the manuscript; Jun‐Bo Yang, Wu‐Xiang Fu, and Zhan‐Xia Ma for help with laboratory work; and Mao‐Sheng Sun and Zhi‐Jia Gu for material collection. This work was funded through projects of the National Natural Science Foundation of China (31070593), the Applied Basic Program of Yunnan (2010CD141), and the Large‐scale Scientific Facilities of the Chinese Academy of Sciences (2009‐LSF‐GBOWS‐01).
Freely available online through the AJB open access option.
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.1200029