Evidence for the Regulation of Gynoecium Morphogenesis by ETTIN via Cell Wall Dynamics

• Published in: Plant physiology (Bethesda) Vol. 178; no. 3; pp. 1222 - 1232
• Main Authors: Andres-Robin, Amélie, Reymond, Mathieu C., Dupire, Antoine, Battu, Virginie, Dubrulle, Nelly, Mouille, Grégory, Lefebvre, Valérie, Pelloux, Jérôme, Boudaoud, Arezki, Traas, Jan, Scutt, Charles P., Monéger, Françoise
• Format: Journal Article
• Language: English
• Published: United States American Society of Plant Biologists 01.11.2018; Oxford University Press ; American Society of Plant Biologists
• Subjects: Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Carboxylic Ester Hydrolases - genetics; Carboxylic Ester Hydrolases - metabolism; Cell Wall - enzymology; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Flowers - genetics; Flowers - growth & development; Flowers - metabolism; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; GENES, DEVELOPMENT AND EVOLUTION; Life Sciences; Meristem - genetics; Meristem - growth & development; Meristem - metabolism; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Pectins - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.18.00745

ETTIN (ETT) is an atypical member of the AUXIN RESPONSE FACTOR family of transcription factors that plays a crucial role in tissue patterning in the Arabidopsis (Arabidopsis thaliana) gynoecium. Though recent insights have provided valuable information on ETT's interactions with other components of auxin signaling, the biophysical mechanisms linking ETT to its ultimate effects on gynoecium morphology were until now unknown. Here, using techniques to assess cell-wall dynamics during gynoecium growth and development, we provide a coherent body of evidence to support a model in which ETT controls the elongation of the valve tissues of the gynoecium through the positive regulation of pectin methylesterase (PME) activity in the cell wall. This increase in PME activity results in an increase in the level of demethylesterified pectins and a consequent reduction in cell wall stiffness, leading to elongation of the valves. Though similar biophysical mechanisms have been shown to act in the stem apical meristem, leading to the expansion of organ primordia, our findings demonstrate that regulation of cell wall stiffness through the covalent modification of pectin also contributes to tissue patterning within a developing plant organ.