Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish

Alcoholic Liver Disease (ALD) refers to damage to the liver due to acute or chronic alcohol abuse. It is among the leading causes of alcohol-related morbidity and mortality and affects more than 2 million people in the United States. A better understanding of the cellular and molecular mechanisms un...

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Published inJournal of visualized experiments no. 123
Main Authors Ellis, Jillian L, Yin, Chunyue
Format Journal Article
LanguageEnglish
Published United States MyJove Corporation 25.05.2017
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Abstract Alcoholic Liver Disease (ALD) refers to damage to the liver due to acute or chronic alcohol abuse. It is among the leading causes of alcohol-related morbidity and mortality and affects more than 2 million people in the United States. A better understanding of the cellular and molecular mechanisms underlying alcohol-induced liver injury is crucial for developing effective treatment for ALD. Zebrafish larvae exhibit hepatic steatosis and fibrogenesis after just 24 h of exposure to 2% ethanol, making them useful for the study of acute alcoholic liver injury. This work describes the procedure for acute ethanol treatment in zebrafish larvae and shows that it causes steatosis and swelling of the hepatic blood vessels. A detailed protocol for Hematoxylin and Eosin (H&E) staining that is optimized for the histological analysis of the zebrafish larval liver, is also described. H&E staining has several unique advantages over immunofluorescence, as it marks all liver cells and extracellular components simultaneously and can readily detect hepatic injury, such as steatosis and fibrosis. Given the increasing usage of zebrafish in modeling toxin and virus-induced liver injury, as well as inherited liver diseases, this protocol serves as a reference for the histological analyses performed in all these studies.
AbstractList Alcoholic Liver Disease (ALD) refers to damage to the liver due to acute or chronic alcohol abuse. It is among the leading causes of alcohol-related morbidity and mortality and affects more than 2 million people in the United States. A better understanding of the cellular and molecular mechanisms underlying alcohol-induced liver injury is crucial for developing effective treatment for ALD. Zebrafish larvae exhibit hepatic steatosis and fibrogenesis after just 24 h of exposure to 2% ethanol, making them useful for the study of acute alcoholic liver injury. This work describes the procedure for acute ethanol treatment in zebrafish larvae and shows that it causes steatosis and swelling of the hepatic blood vessels. A detailed protocol for Hematoxylin and Eosin (H&E) staining that is optimized for the histological analysis of the zebrafish larval liver, is also described. H&E staining has several unique advantages over immunofluorescence, as it marks all liver cells and extracellular components simultaneously and can readily detect hepatic injury, such as steatosis and fibrosis. Given the increasing usage of zebrafish in modeling toxin and virus-induced liver injury, as well as inherited liver diseases, this protocol serves as a reference for the histological analyses performed in all these studies.
Author Yin, Chunyue
Ellis, Jillian L
AuthorAffiliation 2 Division of Developmental Biology, Cincinnati Children's Hospital Medical Center
1 Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children's Hospital Medical Center
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  givenname: Jillian L
  surname: Ellis
  fullname: Ellis, Jillian L
  organization: Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children's Hospital Medical Center
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  givenname: Chunyue
  surname: Yin
  fullname: Yin, Chunyue
  email: chunyue.yin@cchmc.org
  organization: Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children's Hospital Medical Center; Division of Developmental Biology, Cincinnati Children's Hospital Medical Center; chunyue.yin@cchmc.org
BackLink https://www.ncbi.nlm.nih.gov/pubmed/28570521$$D View this record in MEDLINE/PubMed
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Notes Correspondence to: Chunyue Yin at chunyue.yin@cchmc.org
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Snippet Alcoholic Liver Disease (ALD) refers to damage to the liver due to acute or chronic alcohol abuse. It is among the leading causes of alcohol-related morbidity...
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SubjectTerms Animals
Blood Vessels - pathology
Coloring Agents
Developmental Biology
Eosine Yellowish-(YS)
Ethanol - toxicity
Fatty Liver, Alcoholic - pathology
Fatty Liver, Alcoholic - veterinary
Fibrosis
Hematoxylin
Histological Techniques
Larva
Liver - blood supply
Liver - drug effects
Liver - pathology
Zebrafish
Title Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish
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