Dose-dependent effects of corticosteroids on the expression of matrix-related genes in normal and cytokine-treated articular chondrocytes

• Published in: Inflammation research Vol. 52; no. 1; pp. 39 - 49
• Main Authors: Richardson, D. W., Dodge, G. R.
• Format: Journal Article
• Language: English
• Published: Switzerland Springer Nature B.V 01.01.2003
• Subjects: Adrenal Cortex Hormones - pharmacology; Animals; Blotting, Northern; Blotting, Western; Bone Matrix - physiology; Cartilage, Articular; Cells, Cultured; Chondrocytes - drug effects; Chondrocytes - metabolism; Cytokines - pharmacology; Dexamethasone - pharmacology; Dose-Response Relationship, Drug; Ferritins - biosynthesis; Gene Expression Regulation - drug effects; Glucocorticoids - pharmacology; Horses; Humans; Interleukin-1 - pharmacology; Matrix Metalloproteinases - biosynthesis; Matrix Metalloproteinases - genetics; Phenotype; Prednisolone - pharmacology; Protein Biosynthesis; Proteins - genetics; Recombinant Proteins - pharmacology; RNA, Messenger - biosynthesis; Triamcinolone - pharmacology; Tumor Necrosis Factor-alpha - pharmacology

To assess the effects of glucocorticoids on the expression of multiple matrix-related genes in normal and cytokine-treated cultured equine articular chondrocytes in a phenotypically correct suspension culture. Articular cartilage harvested from the joints of 15 foals, 7 yearling horses, and 16 adult...