In vivo GSH depletion induces c-myc expression by modulation of chromatin protein complexes

• Published in: Free radical biology & medicine Vol. 46; no. 11; pp. 1534 - 1542
• Main Authors: Torres, Luis, Sandoval, Juan, Penella, Estela, Zaragozá, Rosa, García, Concha, Rodríguez, José Luis, Viña, Juan R., García-Trevijano, Elena R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2009
• Subjects: Acetylation - drug effects; Animals; Buthionine Sulfoximine - administration & dosage; c-myc; Chromatin Assembly and Disassembly - drug effects; Chromatin remodeling; Free radicals; Gene Expression Regulation; Glutathione - metabolism; GSH; Histone acetylation; Histone Deacetylases - metabolism; Id2; Inhibitor of Differentiation Protein 2 - metabolism; Liver - drug effects; Liver - metabolism; Male; p300-CBP Transcription Factors - metabolism; Phosphorylation; Protein Binding - drug effects; Proto-Oncogene Proteins c-myc - genetics; Proto-Oncogene Proteins c-myc - metabolism; Rats; Rats, Wistar; Repressor Proteins - metabolism; STAT3; STAT3 Transcription Factor - metabolism; Time Factors; Transcriptional Activation - drug effects; Free radicals; c-myc; Id2; ROS; STAT3; PH; Histone acetylation; ChIP; GSH; BSO; HDAC; Chromatin remodeling

We hypothesize that glutathione (GSH) fluctuations could have a prominent role in the modulation of c-myc expression through a mechanism affecting chromatin remodeling complexes. This could lead to an open chromatin structure accessible to transcription factors. We studied the in vivo effect of GSH...