The activation of lymphocyte plasma membrane (Na,K)-ATPase by EGTA is explained better by zinc than calcium chelation

Maximal lymphocyte membrane (Na,K)-ATPase activity occurred only when a chelating agent for divalent cations such as ethylene glycol bis( beta -aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) was present in the reaction mixture. Absence of EGTA, the ionized calcium was 2 mu m and the (...

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Published inThe Journal of biological chemistry Vol. 256; no. 13; pp. 6629 - 6632
Main Authors Segel, G B, Simon, W, Lichtman, A H, Lichtman, M A
Format Journal Article
LanguageEnglish
Published United States 10.07.1981
Subjects
adenosinetriphosphatase
Calcium - pharmacology
Cell Membrane - enzymology
Egtazic Acid - pharmacology
Enzyme Activation
ethylene glycol bis( beta -aminoethyl ether)-N,N,N'N'-tetraacetic acid
Ethylene Glycols - pharmacology
Humans
Kinetics
lymphocytes
Lymphocytes - enzymology
Mathematics
Sodium-Potassium-Exchanging ATPase - metabolism
Zinc - pharmacology
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Summary:Maximal lymphocyte membrane (Na,K)-ATPase activity occurred only when a chelating agent for divalent cations such as ethylene glycol bis( beta -aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) was present in the reaction mixture. Absence of EGTA, the ionized calcium was 2 mu m and the (Na,K)-ATPase was 25 to 40% of maximum. The data established that enhancement of the activity of the lymphocyte (Na,K)-ATPase by EGTA is not explained by chelation of Ca; rather, the chelation of Zn activates the (Na,K)-ATPase under the conditions of these studies.
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ISSN:0021-9258
DOI:10.1016/S0021-9258(19)69036-8