Isolation of human mesenchymal stem cells and their cultivation on the porous bone matrix

• Published in: Journal of visualized experiments no. 96; p. e51999
• Main Authors: Rodríguez-Fuentes, Nayeli, Reynoso-Ducoing, Olivia, Rodríguez-Hernández, Ana, Ambrosio-Hernández, Javier R, Piña-Barba, Maria C, Zepeda-Rodríguez, Armando, Cerbón-Cervantes, Marco A, Tapia-Ramírez, José, Alcantara-Quintana, Luz E
• Format: Journal Article
• Language: English
• Published: United States MyJove Corporation 09.02.2015
• Subjects: Amnion - cytology; Animals; Biocompatible Materials; Bone Matrix; Cattle; Cell Adhesion - physiology; Cell Culture Techniques - methods; Cell Lineage; Developmental Biology; Guided Tissue Regeneration - methods; Humans; Mesenchymal Stromal Cells - cytology; Osteoblasts - cytology; Tissue Scaffolds

Mesenchymal stem cells (MSCs) have a differentiation potential towards osteoblastic lineage when they are stimulated with soluble factors or specific biomaterials. This work presents a novel option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) that employs bovine bone matrix Nukbo...