Occurrence of Pandemic Clones of Vibrio parahaemolyticus Isolates from Seafood and Clinical Samples in a Chinese Coastal Province

Fifty-four isolates of Vibrio parahaemolyticus were examined for hemolytic and urease-producing phenotypes as well as presence of virulence markers by polymerase chain reaction (PCR). All clinical isolates (11/11, 100%) and one out of 42 isolates from seafood (2.4%) possessed the tdh gene and showed...

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Published inFoodborne pathogens and disease Vol. 5; no. 2; pp. 127 - 134
Main Authors Vongxay, Khamphouth, Pan, Zijiang, Zhang, Xiaofeng, Wang, Shuna, Cheng, Suyun, Mei, Lingling, Xu, Cheng, Fang, Weihuan
Format Journal Article
LanguageEnglish
Published United States 01.04.2008
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Abstract Fifty-four isolates of Vibrio parahaemolyticus were examined for hemolytic and urease-producing phenotypes as well as presence of virulence markers by polymerase chain reaction (PCR). All clinical isolates (11/11, 100%) and one out of 42 isolates from seafood (2.4%) possessed the tdh gene and showed hemolysis. This tdh-positive seafood isolate as well as four clinical isolates belonged to the new pandemic clone O3:K6 according to serotyping and sequencing of the toxRS locus. The new O3:K6 clone, O1:KUT, and O4:K68 shared the same characteristic variations from the old O3:K6 clone at six base positions from 576 to 1244 of the toxRS locus. Seven clinical isolates (63.6%) were positive on both toxRS- and orf8-based PCRs. Our results indicate that the new pandemic O3:K6 clonal group of V. parahaemolyticus isolates from clinical and seafood sources are present in the southeastern coastal Chinese province Zhejiang and the pandemic clones of V. parahaemolyticus should be targeted for control of seafood-related transmission to humans.
AbstractList Fifty-four isolates of Vibrio parahaemolyticus were examined for hemolytic and urease-producing phenotypes as well as presence of virulence markers by polymerase chain reaction (PCR). All clinical isolates (11/11, 100%) and one out of 42 isolates from seafood (2.4%) possessed the tdh gene and showed hemolysis. This tdh-positive seafood isolate as well as four clinical isolates belonged to the new pandemic clone O3:K6 according to serotyping and sequencing of the toxRS locus. The new O3:K6 clone, O1:KUT, and O4:K68 shared the same characteristic variations from the old O3:K6 clone at six base positions from 576 to 1244 of the toxRS locus. Seven clinical isolates (63.6%) were positive on both toxRS- and orf8-based PCRs. Our results indicate that the new pandemic O3:K6 clonal group of V. parahaemolyticus isolates from clinical and seafood sources are present in the southeastern coastal Chinese province Zhejiang and the pandemic clones of V. parahaemolyticus should be targeted for control of seafood-related transmission to humans.
Author Fang, Weihuan
Wang, Shuna
Cheng, Suyun
Mei, Lingling
Xu, Cheng
Zhang, Xiaofeng
Pan, Zijiang
Vongxay, Khamphouth
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Snippet Fifty-four isolates of Vibrio parahaemolyticus were examined for hemolytic and urease-producing phenotypes as well as presence of virulence markers by...
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StartPage 127
SubjectTerms Animals
bacterial contamination
bacterial infections
Bacterial Proteins - genetics
Base Sequence
clones
Disease Outbreaks
disease prevalence
disease transmission
DNA-Binding Proteins - genetics
Drug Resistance, Bacterial
food contamination
Food Contamination - analysis
food pathogens
food quality
foodborne illness
Genetic Markers
Hemolysin Proteins - genetics
hemolysis
human health
Humans
mathematical models
Membrane Proteins - genetics
Molecular Sequence Data
nucleotide sequences
pandemic
phenotype
polymerase chain reaction
Polymerase Chain Reaction - methods
Risk Assessment
Seafood - microbiology
seafoods
Serotyping
Shellfish - microbiology
Transcription Factors - genetics
urease
Vibrio Infections - microbiology
Vibrio Infections - transmission
Vibrio parahaemolyticus
Vibrio parahaemolyticus - classification
Vibrio parahaemolyticus - genetics
Vibrio parahaemolyticus - isolation & purification
Vibrio parahaemolyticus - pathogenicity
virulence
Virulence - genetics
Title Occurrence of Pandemic Clones of Vibrio parahaemolyticus Isolates from Seafood and Clinical Samples in a Chinese Coastal Province
URI https://www.ncbi.nlm.nih.gov/pubmed/18370608
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