Assay for the simultaneous determination of acetaminophen–caffeine–butalbital in human serum using a monolithic column

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 36; no. 4; pp. 737 - 741
• Main Authors: Pistos, C., Stewart, J.T.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 19.11.2004; Elsevier Science
• Subjects: Acetaminophen - blood; Acetaminophen - chemistry; Acetaminophen–caffeine–butalbital; Analysis; Analytical, structural and metabolic biochemistry; Barbiturates - blood; Barbiturates - chemistry; Biological and medical sciences; Caffeine - blood; Caffeine - chemistry; Chromatography, High Pressure Liquid - methods; Drug Combinations; Fundamental and applied biological sciences. Psychology; General pharmacology; HPLC; Humans; Medical sciences; Monolithic column; Pharmacology. Drug treatments; Serum; Serum; Acetaminophen–caffeine–butalbital; HPLC; Monolithic column; Human; Acetaminophen-caffeine-butalbital; Biological fluid; CNS stimulant; Psychotropic; Antimigrainous agent; HPLC chromatography; Hypnotic; Respiratory analeptic; Butalbital; Paracetamol; Analgesic; Simultaneous measurement; Antipyretic; Xanthine derivatives; Sedative; Barbiturates; Caffeine

A fast and sensitive high performance liquid chromatography (HPLC) assay was developed on a C18 monolithic column for the simultaneous determination of acetaminophen–caffeine–butalbital in human serum. Serum samples were treated with a solid phase extraction procedure. The analytes were separated us...