Selective Analysis of Dopamine Receptor Antagonist LE300 and its N-Methyl Metabolite in Mouse Sera at the Trace Level by HPLC–Fluorescence Detection

• Published in: Chromatographia Vol. 78; no. 9-10; pp. 655 - 661
• Main Authors: Hefnawy, Mohamed, Alanazi, Amer, Abounassif, Mohammed, Mohammed, Mostafa, Al-Swaidan, Ibrahim, Attia, Sabry, Mostafa, Gamal, El-Subbagh, Hussein, Lehmann, Jochen
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.05.2015; Springer Berlin Heidelberg
• Subjects: Analytical Chemistry; antagonists; Chemistry; Chemistry and Materials Science; Chromatography; detection limit; dopamine receptors; guidelines; high performance liquid chromatography; Laboratory Medicine; metabolites; mice; Original; pharmacokinetics; Pharmacy; phosphates; Proteomics; regression analysis; verapamil; wavelengths; Trace analysis; Pharmacokinetics; LE300; HPLC; Fluorescence detection

A highly selective, sensitive, and reliable high-performance liquid chromatography (HPLC) method was developed and validated for the simultaneous determination of a novel type of dopamine receptor antagonist LE300 and its N-methyl metabolite in mouse sera. LE300, its N-methyl metabolite, and verapam...