Analysis of hypermethylation in the RPS element suggests a signal function for short inverted repeats in de novo methylation

• Published in: Plant molecular biology Vol. 48; no. 4; pp. 383 - 399
• Main Authors: Müller, Andreas, Marins, Mozart, Kamisugi, Yasuko, Meyer, Peter
• Format: Journal Article
• Language: English
• Published: Netherlands Springer Nature B.V 01.03.2002
• Subjects: Animals; Arabidopsis; Arabidopsis thaliana; Base Sequence; Binding Sites; Blotting, Southern; chromosomes; Deoxyribonucleases, Type II Site-Specific; Deoxyribonucleases, Type II Site-Specific - metabolism; Deoxyribonucleic acid; DNA; DNA - genetics; DNA - metabolism; DNA conformation; DNA Methylation; DNA, Plant; DNA, Plant - genetics; DNA, Plant - metabolism; Evolution, Molecular; Gene Expression Regulation, Plant; Genes; genetic transformation; Genetic Variation; genetics; Genome, Plant; Heterochromatin; Heterochromatin - genetics; Humans; In Situ Hybridization, Fluorescence; metabolism; methylation; Models, Genetic; Molecular Sequence Data; Nicotiana - genetics; nucleic acid hybridization; Petunia hybrida; Plants; Plants - genetics; protein secondary structure; repetitive sequences; Repetitive Sequences, Nucleic Acid; Repetitive Sequences, Nucleic Acid - genetics; Solanaceae; Solanaceae - genetics; Species Specificity; Tobacco; transgenic plants

A repetitive DNA sequence (RPS) from Petunia hybrida had previously been shown to enhance expression variegation in petunia and tobacco and to carry a hot spot for de novo DNA methylation. Here we show that a strong de novo hypermethylation site is located within a palindromic segment of the RPS and...