Pseudomonas defluvii sp. nov., isolated from hospital sewage

• Published in: International journal of systematic and evolutionary microbiology Vol. 70; no. 7; pp. 4199 - 4203
• Main Authors: Qin, Jiayuan, Hu, Yiyi, Wu, Wenjing, Feng, Yu, Zong, Zhiyong
• Format: Journal Article
• Language: English
• Published: 01.07.2020
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.004272

A novel Gram-negative, obligate aerobic, mobile, rod-shaped and non-spore-forming bacterial strain, WCHP16 T , was isolated from the wastewater treatment plant at West China Hospital in Chengdu, PR China. It was characterized using a polyphasic approach. Analysis of its 16S rRNA gene sequence showed that strain WCHP16 T belonged to the genus Pseudomonas with the highest similarity to Pseudomonas qingdaonensis JJ3 T (99.34 %), Pseudomonas shirazica VM14 T (99.0 %), Pseudomonas plecoglossicida NBRC 103162 T (99.0 %) and Pseudomonas asiatica RYU5 T (99.0 %). Phylogenomic analysis based on 107 core gene sequences demonstrated that WCHP16 T was a member of the Pseudomonas putida group but was distant from all closely related species. Whole-genome comparisons, using average nucleotide identity based on blast (ANIb) and in silico DNA–DNA hybridization ( is DDH), confirmed low genome relatedness to all the known Pseudomonas species (below the recommended thresholds of 95 % [ANIb] and 70 % [ is DDH] for species delineation). Phenotypic characterization tests showed that the utilization of phenylacetic acid and capric acid, but not d -arabitol, and inability to produce fluorescent (King B medium) in combination could distinguish this strain from other related species of the genus Pseudomonas . Therefore, based on genetic and phenotypic evidence, it is clear that strain WCHP16 T represents a novel species, for which the name Pseudomonas defluvii sp. nov. is proposed. The type strain is WCHP16 T (GDMCC1.1215 T =CCTCC AB 2017103 T =KCTC 52991 T ).