Pseudomonas defluvii sp. nov., isolated from hospital sewage
A novel Gram-negative, obligate aerobic, mobile, rod-shaped and non-spore-forming bacterial strain, WCHP16 T , was isolated from the wastewater treatment plant at West China Hospital in Chengdu, PR China. It was characterized using a polyphasic approach. Analysis of its 16S rRNA gene sequence showed...
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Published in | International journal of systematic and evolutionary microbiology Vol. 70; no. 7; pp. 4199 - 4203 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
01.07.2020
|
Online Access | Get full text |
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Summary: | A novel Gram-negative, obligate aerobic, mobile, rod-shaped and non-spore-forming bacterial strain, WCHP16
T
, was isolated from the wastewater treatment plant at West China Hospital in Chengdu, PR China. It was characterized using a polyphasic approach. Analysis of its 16S rRNA gene sequence showed that strain WCHP16
T
belonged to the genus
Pseudomonas
with the highest similarity to
Pseudomonas qingdaonensis
JJ3
T
(99.34 %),
Pseudomonas shirazica
VM14
T
(99.0 %),
Pseudomonas plecoglossicida
NBRC 103162
T
(99.0 %) and
Pseudomonas asiatica
RYU5
T
(99.0 %). Phylogenomic analysis based on 107 core gene sequences demonstrated that WCHP16
T
was a member of the
Pseudomonas putida
group but was distant from all closely related species. Whole-genome comparisons, using average nucleotide identity based on
blast
(ANIb) and
in silico
DNA–DNA hybridization (
is
DDH), confirmed low genome relatedness to all the known
Pseudomonas
species (below the recommended thresholds of 95 % [ANIb] and 70 % [
is
DDH] for species delineation). Phenotypic characterization tests showed that the utilization of phenylacetic acid and capric acid, but not
d
-arabitol, and inability to produce fluorescent (King B medium) in combination could distinguish this strain from other related species of the genus
Pseudomonas
. Therefore, based on genetic and phenotypic evidence, it is clear that strain WCHP16
T
represents a novel species, for which the name
Pseudomonas defluvii
sp. nov. is proposed. The type strain is WCHP16
T
(GDMCC1.1215
T
=CCTCC AB 2017103
T
=KCTC 52991
T
). |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1466-5026 1466-5034 |
DOI: | 10.1099/ijsem.0.004272 |