Thin-layer agarose isoelectric focusing of alkaline phosphatase isoenzymes from human neutrophils

• Published in: Electrophoresis Vol. 9; no. 5; p. 221
• Main Authors: Brisson-Lougarre, A, Vergnes, H, Grozdea, J, Biermé, R
• Format: Journal Article
• Language: English
• Published: Germany 1988
• Subjects: Alkaline Phosphatase - analysis; Detergents; Isoelectric Focusing; Isoenzymes - analysis; Neutrophils - enzymology; Solubility; Sonication; Staining and Labeling

An improved method for thin-layer agarose isoelectric focusing of alkaline phosphatases (AP) from human neutrophils is described. The solubilization of AP isoenzymes was studied with four detergents. The best results were obtained after sonication with Zwittergent 3-12 (1% final concentration), foll...