Eliciting macroporosity in polyacrylamide and agarose gels with polyethylene glycol

Preparation of highly porous polyacrylamide has recently been described (Righetti et al., Electrophoresis, 1992, 13, 587-595). In this report we add new observations on the conditions of promoting macroporosity in polyacrylamide gels and extend the possibility of eliciting this phenomenon to agarose...

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Bibliographic Details
Published inElectrophoresis Vol. 17; no. 1; p. 58
Main Authors Charlionet, R, Levasseur, L, Malandain, J J
Format Journal Article
LanguageEnglish
Published Germany 01.01.1996
Subjects
Acrylic Resins - chemistry
Gels - chemistry
Microscopy, Electron, Scanning
Polyethylene Glycols
Porosity
Sepharose - chemistry
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Summary:Preparation of highly porous polyacrylamide has recently been described (Righetti et al., Electrophoresis, 1992, 13, 587-595). In this report we add new observations on the conditions of promoting macroporosity in polyacrylamide gels and extend the possibility of eliciting this phenomenon to agarose matrices by the combined use of polyethylene glycol and glycerol. The process of cluster formation in hot agarose solutions was studied and gel structures were examined by scanning electron microscopy. A definition of macroporosity in gel, related to controlled microsyneresis during gelation, is tentatively proposed. The unexpected influence of acrylamide and agarose concentrations upon the size of macroporous structures in the corresponding gels is revealed.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.1150170111