The alkyne-tag Raman imaging of coronatine, a plant pathogen virulence factor, in Commelina communis and its possible mode of action

• Published in: Organic & biomolecular chemistry Vol. 16; no. 18; pp. 3348 - 3352
• Main Authors: Ueda, Minoru, Hayashi, Kengo, Egoshi, Syusuke, Ishimaru, Yasuhiro, Takaoka, Yousuke, Yamakoshi, Hiroyuki, Dodo, Kosuke, Sodeoka, Mikiko
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 2018
• Subjects: Alkynes; Alkynes - chemistry; Amino Acids - analysis; Arabidopsis - chemistry; Arabidopsis - microbiology; Bacterial diseases; Bacterial Toxins - analysis; Commelina - chemistry; Commelina - microbiology; Coronatine; Endoplasmic reticulum; Endoplasmic Reticulum - chemistry; Endoplasmic Reticulum - microbiology; Indenes - analysis; Localization; Mode of action; Molecular Docking Simulation; Plant Diseases - microbiology; Signal transduction; Spectrum Analysis, Raman - methods; Virulence; Virulence factors
• ISSN: 1477-0520; 1477-0539; 1477-0539
• DOI: 10.1039/C8OB00097B

We previously reported that coronatine, a virulence factor of plant bacteria, facilitates bacterial infection through an ER (endoplasmic reticulum)-mediated, non-canonical mechanism in the model dicot plant, Arabidopsis thaliana . Here, we report that this same ER-mechanism is ubiquitous among dicots and monocots, and works by affecting the ethylene signaling pathway widely found in plants. The subcellular localization of coronatine by the alkyne-tag Raman imaging (ATRI) approach provided a convincing clue.