An ATP dependent reduction of nitrate to ammonia by a cell free particulate system from barley roots

• Published in: Biochemical and biophysical research communications Vol. 40; no. 6; pp. 1305 - 1310
• Main Authors: Bourne, W.F., Miflin, B.J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.09.1970
• Subjects: Adenosine Triphosphate - pharmacology; Ammonia - metabolism; botany; Cell-Free System; Chromatography, Gel; Edible Grain - cytology; Edible Grain - drug effects; Edible Grain - enzymology; Edible Grain - metabolism; Electron Transport; Kinetics; Nitrates - metabolism; Nitrites - metabolism; Oxidation-Reduction; Plant Cells; Plant Science and Plant Products; plants; Plants - drug effects; Plants - enzymology; Plants - metabolism; Quaternary Ammonium Compounds - metabolism; Stimulation, Chemical; Vibration
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(70)90008-2

A cell free, particulate extract of barley roots has been shown to be capable of producing ammonia from nitrate solely in the presence of compounds known to be in the root. This reaction is dependent on added ATP and to a lesser extent on electron donors. Sonication and Sephadex G25 treatment of the particulate fraction yields a preparation in which ammonia production is nitrate dependent. Studies of changes in the levels of nitrate, nitrite and ammonia indicate that ATP is required for the reduction of nitrite to ammonia