Biomimetic, Smart, and Multivalent Ligands for G-Quadruplex Isolation and Bioorthogonal Imaging

G-quadruplexes (G4s) continue to gather wide attention in the field of chemical biology as their prevalence in the human genome and transcriptome strongly suggests that they play key regulatory roles in cell biology. G4-specific, cell-permeable small molecules (G4-ligands) innovatively permit the in...

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Published inACS chemical biology Vol. 16; no. 5; pp. 905 - 914
Main Authors Sperti Rota, Francesco, Charbonnier, Thibaut, Lejault, Pauline, Zell, Joanna, Bernhard, Claire, Valverde, Ibai E, Monchaud, David
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 21.05.2021
Subjects
Biochemistry, Molecular Biology
Biomimetic Materials - chemical synthesis
Biomimetic Materials - isolation & purification
Biophysics
Biotinylation
Cell Membrane Permeability
Chemical Sciences
Click Chemistry
G-Quadruplexes
Humans
Life Sciences
Ligands
MCF-7 Cells
Molecular Structure
Nucleic Acids - chemistry
Optical Imaging
Organic chemistry
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Abstract G-quadruplexes (G4s) continue to gather wide attention in the field of chemical biology as their prevalence in the human genome and transcriptome strongly suggests that they play key regulatory roles in cell biology. G4-specific, cell-permeable small molecules (G4-ligands) innovatively permit the interrogation of cellular circuitries in order to assess to what extent G4s influence cell fate and functions. Here, we report on multivalent, biomimetic G4-ligands referred to as TASQs that enable both the isolation and visualization of G4s in human cells. Two biotinylated TASQs, and , are indeed efficient molecular tools to isolate G4s from mixtures of nucleic acids through simple affinity capture protocols and to image G4s in cells via a biotin/avidin pretargeted imaging system first applied here to G4s, found to be a reliable alternative to in situ click chemistry.
AbstractList G-quadruplexes (G4s) continue to gather wide attention in the field of chemical biology as their prevalence in the human genome and transcriptome strongly suggests that they play key regulatory roles in cell biology. G4-specific, cell-permeable small molecules (G4-ligands) innovatively permit the interrogation of cellular circuitries in order to assess to what extent G4s influence cell fate and functions. Here, we report on multivalent, biomimetic G4-ligands referred to as TASQs that enable both the isolation and visualization of G4s in human cells. Two biotinylated TASQs, BioTASQ and BioCyTASQ, are indeed efficient molecular tools to isolate G4s from mixtures of nucleic acids through simple affinity capture protocols and to image G4s in cells via a biotin/avidin pretargeted imaging system first applied here to G4s, found to be a reliable alternative to in situ click chemistry.
G-quadruplexes (G4s) continue to gather wide attention in the field of chemical biology as their prevalence in the human genome and transcriptome strongly suggests that they play key regulatory roles in cell biology. G4-specific, cell-permeable small molecules (G4-ligands) innovatively permit the interrogation of cellular circuitries in order to assess to what extent G4s influence cell fate and functions. Here, we report on multivalent, biomimetic G4-ligands referred to as TASQs that enable both the isolation and visualization of G4s in human cells. Two biotinylated TASQs, and , are indeed efficient molecular tools to isolate G4s from mixtures of nucleic acids through simple affinity capture protocols and to image G4s in cells via a biotin/avidin pretargeted imaging system first applied here to G4s, found to be a reliable alternative to in situ click chemistry.
Author Zell, Joanna
Charbonnier, Thibaut
Sperti Rota, Francesco
Lejault, Pauline
Valverde, Ibai E
Monchaud, David
Bernhard, Claire
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Snippet G-quadruplexes (G4s) continue to gather wide attention in the field of chemical biology as their prevalence in the human genome and transcriptome strongly...
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SubjectTerms Biochemistry, Molecular Biology
Biomimetic Materials - chemical synthesis
Biomimetic Materials - isolation & purification
Biophysics
Biotinylation
Cell Membrane Permeability
Chemical Sciences
Click Chemistry
G-Quadruplexes
Humans
Life Sciences
Ligands
MCF-7 Cells
Molecular Structure
Nucleic Acids - chemistry
Optical Imaging
Organic chemistry
Title Biomimetic, Smart, and Multivalent Ligands for G-Quadruplex Isolation and Bioorthogonal Imaging
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