Surface optimization of gold nanoparticle mass tags for the sensitive detection of protein biomarkers via immuno-capture LI-MS

HPV-induced cervical cancer is one of the most lethal cancers. Therefore, the development of a reliable and accurate method for the early diagnosis of HPV infections is highly important. Here, gold nanoparticles (AuNPs) were utilized as mass tags in an immuno-capture LI-MS assay for the detection of...

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Published inAnalyst (London) Vol. 145; no. 19; pp. 6237 - 6242
Main Authors Tam, Toby Siu-Chung, Cheng, Yu-Hong, Lok, Chun-Nam, Au-Yeung, Ho Yu, Ni, Wen-Xiu, Wei, Xiao-Long, Ng, Kwan-Ming
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 28.09.2020
Subjects
Antibodies
Antigens
Biomarkers
Cancer
Gold
HeLa Cells
Human papillomavirus
Humans
Metal Nanoparticles
Nanoparticles
Optimization
Proteins
Tags
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Summary:HPV-induced cervical cancer is one of the most lethal cancers. Therefore, the development of a reliable and accurate method for the early diagnosis of HPV infections is highly important. Here, gold nanoparticles (AuNPs) were utilized as mass tags in an immuno-capture LI-MS assay for the detection of HPV marker proteins. Through the optimization of the amount of antibodies and surface charges on AuNPs, high antigen detection efficiency with minimal non-specific binding was achieved. With optimized antibody-conjugated AuNPs, low attomole amount of HPV proteins in HeLa cell lysate was quantified.
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ISSN:0003-2654
1364-5528
DOI:10.1039/d0an01121e