Immunoproteomics of membrane proteins of Shigella flexneri 2a 2457T

• Published in: World journal of gastroenterology : WJG Vol. 11; no. 43; pp. 6880 - 6883
• Main Authors: Ying, Tian-Yi, Wang, Jun-Jun, Wang, Heng-Liang, Feng, Er-Ling, Wei, Kai-Hua, Huang, Liu-Yu, Huang, Pei-Tang, Huang, Cui-Fen
• Format: Journal Article
• Language: English
• Published: United States Beijing Institute of Pharmaceutical Chemistry,Beijing 102205,China%Beijing Institute of Biotechnology,State Key Lab of Pathogen and Biosecurity,Beijing 100071,China 21.11.2005; Beijing Institute of Biotechnology,State Key Lab of Pathogen and Biosecurity,Beijing 100071,China; Baishideng Publishing Group Inc
• Subjects: Animals; Antigens, Bacterial - chemistry; Antigens, Bacterial - immunology; Bacterial Proteins - chemistry; Bacterial Proteins - immunology; Child; Humans; Membrane Proteins - chemistry; Membrane Proteins - immunology; Proteomics - methods; Rabbits; Rapid Communication; Shigella flexneri - immunology; Immunoproteomics; Shigella flexneri 2a 2457T; Membrane proteins
• DOI: 10.3748/wjg.v11.i43.6880
• ISSN: 1007-9327

To screen the immunogenic membrane proteins of Shigella flexneri 2a 2457T. The routine two-dimensional polyacrylamide gel electrophoresis (2-DE) and Western blotting were combined to screen immunogenic proteins of S. flexneri 2a 2457T. Serum was gained from rabbits immunized with the same bacteria. Immunogenic spots were cut out from the polyacrylamide gel and digested by trypsin in-gel. Matrix-assisted laser desorption/ionization time of flight-mass spectrometry (MALDI-TOF-MS) was performed to determine the molecular weight of peptides. Electrospray ionization (ESI-MS/MS) was performed to determine the sequences of the interesting peptides. A total of 20 spots were successfully identified from Coomassie brilliant blue stained gels representing 13 protein entries, 5 known antigens and 8 novel antigens. A hypothetical protein (YaeT) was detected, which might be a candidate target of vaccine. Membrane proteins of S. flexneri 2a 2457T were successfully observed by 2-DE. Several known and novel antigens were identified by mass spectrum.