Immunoproteomics of membrane proteins of Shigella flexneri 2a 2457T
To screen the immunogenic membrane proteins of Shigella flexneri 2a 2457T. The routine two-dimensional polyacrylamide gel electrophoresis (2-DE) and Western blotting were combined to screen immunogenic proteins of S. flexneri 2a 2457T. Serum was gained from rabbits immunized with the same bacteria....
Saved in:
Published in | World journal of gastroenterology : WJG Vol. 11; no. 43; pp. 6880 - 6883 |
---|---|
Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Beijing Institute of Pharmaceutical Chemistry,Beijing 102205,China%Beijing Institute of Biotechnology,State Key Lab of Pathogen and Biosecurity,Beijing 100071,China
21.11.2005
Beijing Institute of Biotechnology,State Key Lab of Pathogen and Biosecurity,Beijing 100071,China Baishideng Publishing Group Inc |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | To screen the immunogenic membrane proteins of Shigella flexneri 2a 2457T.
The routine two-dimensional polyacrylamide gel electrophoresis (2-DE) and Western blotting were combined to screen immunogenic proteins of S. flexneri 2a 2457T. Serum was gained from rabbits immunized with the same bacteria. Immunogenic spots were cut out from the polyacrylamide gel and digested by trypsin in-gel. Matrix-assisted laser desorption/ionization time of flight-mass spectrometry (MALDI-TOF-MS) was performed to determine the molecular weight of peptides. Electrospray ionization (ESI-MS/MS) was performed to determine the sequences of the interesting peptides.
A total of 20 spots were successfully identified from Coomassie brilliant blue stained gels representing 13 protein entries, 5 known antigens and 8 novel antigens. A hypothetical protein (YaeT) was detected, which might be a candidate target of vaccine.
Membrane proteins of S. flexneri 2a 2457T were successfully observed by 2-DE. Several known and novel antigens were identified by mass spectrum. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Telephone: +86-10-66948836 Correspondence to: Dr Heng-Liang Wang, Institute of Biotechnology, State Key Lab of Pathogen and Biosecurity, 20 Dongdajie Street, Fengtai District, Beijing 100071, China. wanghl@nic.bmi.ac.cn Author contributions: All authors contributed equally to the work. |
ISSN: | 1007-9327 2219-2840 |
DOI: | 10.3748/wjg.v11.i43.6880 |