A membrane-bound alkaline inorganic pyrophosphatase in isolated spinach chloroplasts

• Published in: Archives of biochemistry and biophysics Vol. 154; no. 2; pp. 606 - 613
• Main Authors: Gould, J.Michael, Winget, G.Douglas
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.1973
• Subjects: Adenosine Triphosphate - pharmacology; Alkalies; Chlorophyll - analysis; Chloroplasts - drug effects; Chloroplasts - enzymology; Diphosphates - analysis; horticultural crops; Hydrogen-Ion Concentration; Hydrolysis; In Vitro Techniques; Kinetics; Magnesium; Membranes - enzymology; Phosphorus Isotopes; plant biochemistry; Plant Cells; plant physiology; Pyrophosphatases - analysis; Spectrophotometry

An alkaline inorganic pyrophosphatase is found in association with isolated spinach chloroplast membranes. The enzyme is not removed from chloroplasts by repeated washings in an iso-osmotic medium. Suspension of the chloroplasts in hyper- or hypo-osmotic medium, however, results in the loss of pyrop...