Dietary alpha- and gamma-tocopherol (1:5 ratio) supplementation attenuates adipose tissue expansion, hepatic steatosis, and expression of inflammatory markers in a high-fat-diet–fed murine model

• Published in: Nutrition (Burbank, Los Angeles County, Calif.) Vol. 85; p. 111139
• Main Authors: Juretić, Nevenka, Sepúlveda, Ruth, D'Espessailles, Amanda, Vera, Daniela B., Cadagan, Cynthia, de Miguel, Manuel, González-Mañán, Daniel, Tapia, Gladys
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2021; Elsevier Limited
• Subjects: Adipocytes; Adipose tissue; Animal models; Attenuation; Biomarkers; Body fat; Body weight; Carbohydrates; Cytokines; Diet; Dietary supplements; Drug dosages; Enlargement; Fatty acids; Fatty liver; Gene expression; Hepatic steatosis; High fat diet; IL-1β; Inflammation; Interleukin 1; Laboratory animals; Lipid metabolism; Lipids; Liver; Liver diseases; Metabolism; Microscopy; NF-κB protein; Obesity; Oxidative stress; Peroxisome proliferator-activated receptors; Polymerase chain reaction; Proteins; Steatosis; Tocopherol; Triglycerides; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Vitamin E; United States > US; Obesity; Inflammation; Hepatic steatosis; Lipid metabolism; Tocopherol
• DOI: 10.1016/j.nut.2021.111139
• ISSN: 0899-9007

The aim of this study was to evaluate the effect of the dietary supplementation of an alpha- and gamma-tocopherol mixture (1:5 ratio) in the adipose tissue expansion, hepatic steatosis, and expression of inflammatory markers induced by consumption of a high-fat diet (HFD) in mice. Male C57BL/6 J mice were fed for 12 wk and divided into the following: 1) control diet (CD; 10% fat, 20% protein, 70% carbohydrates); 2) CD + TF (CD plus alpha-tocopherol: 0.7 mg/kg/d, gamma-tocopherol: 3.5 mg/kg/d); 3) HFD (60% fat, 20% protein, 20% carbohydrates); and 4) HFD + TF (HFD plus alpha-tocopherol: 0.7 mg/kg/d, gamma-tocopherol: 3.5 mg/kg/d). General parameters, adipocyte size, liver steatosis, adipose and hepatic tumor necrosis factor-α (TNF-α) and interleukin-1 β (IL-1β) expression, hepatic nuclear factor kappa B (NF-κB), and peroxisome proliferator-activated receptor α (PPAR-α) levels were evaluated. Tocopherol supplementation in HFD-fed mice showed a significant decrease in the body weight (19%) and adipose tissue weight (52%), adipose tissue/body weight ratio (36%), and serum triacylglycerols (56%); a 42% decrease (P < 0.05) of adipocyte size compared to HFD; attenuation of liver steatosis by decreasing (P < 0.05) lipid vesicles presence (90%) and total lipid content (75%); and downregulation of inflammatory markers (TNF-α and IL-1β), along with an upregulation of hepatic PPAR-α expression and its downstream-regulated genes (ACOX and CAT-1), and an inhibition of hepatic NF-κB activation. The present study suggests that alpha- and gamma-tocopherol (1:5 ratio) supplementation attenuates the adipocyte enlargement, hepatic steatosis, and metabolic inflammation induced by HFD in association with PPAR-α/NF-κB modulation. •Dietary alpha- and gamma-tocopherol (1:5 ratio) supplementation attenuates adipose tissue expansion.•Dietary alpha- and gamma-tocopherol (1:5 ratio) supplementation prevents hepatic steatosis induced by a high-fat diet.•Dietary alpha- and gamma-tocopherol (1:5 ratio) supplementation upregulates hepatic peroxisome proliferator-activated receptor α activity.