Internally controlled RNA sequencing comparisons using nucleoside recoding chemistry

• Published in: Nucleic acids research Vol. 50; no. 19; p. e110
• Main Authors: Courvan, Meaghan C S, Niederer, Rachel O, Vock, Isaac W, Kiefer, Lea, Gilbert, Wendy V, Simon, Matthew D
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 28.10.2022
• Subjects: Methods Online; Nucleosides; RNA - chemistry; RNA, Messenger - metabolism; Sequence Analysis, RNA; Thiouridine - chemistry

Quantitative comparisons of RNA levels from different samples can lead to new biological understanding if they are able to distinguish biological variation from variable sample preparation. These challenges are pronounced in comparisons that require complex biochemical manipulations (e.g. isolating...