In situ multiplex detection of serum exosomal microRNAs using an all-in-one biosensor for breast cancer diagnosis

• Published in: Analyst (London) Vol. 145; no. 9; pp. 3289 - 3296
• Main Authors: Wang, Huizhen, He, Dinggeng, Wan, Kejing, Sheng, Xiaowu, Cheng, Hong, Huang, Jin, Zhou, Xiao, He, Xiaoxiao, Wang, Kemin
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 07.05.2020
• Subjects: Biosensing Techniques - methods; Biosensors; Breast; Breast cancer; Breast Neoplasms - diagnosis; Breast Neoplasms - genetics; Breast Neoplasms - pathology; Cell Line; Chemical compounds; Diagnosis; Donors (electronic); Electrophoresis, Polyacrylamide Gel; Exosomes - metabolism; Female; Fluorescence; Fluorescent Dyes - chemistry; Gene sequencing; Humans; Limit of Detection; MCF-7 Cells; MicroRNAs; MicroRNAs - blood; Multiplexing; Oligonucleotides; Recognition

Herein, a simple all-in-one biosensor based on a DNA three-way junction has been constructed for in situ simultaneous detection of multiple miRNAs by competitive strand displacement. In our design, three oligonucleotides (Y1, Y2 and Y3) of a Y-type scaffold were extended at their 5′ ends by introduc...