Circularized synthetic oligodeoxynucleotides serve as promoterless RNA polymerase III templates for small RNA generation in human cells

• Published in: Nucleic acids research Vol. 41; no. 4; pp. 2552 - 2564
• Main Authors: Seidl, Christine I, Lama, Lodoe, Ryan, Kevin
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.02.2013
• Subjects: DNA, Circular - chemistry; HEK293 Cells; Humans; Nucleic Acid Conformation; Oligodeoxyribonucleotides - chemistry; Oligodeoxyribonucleotides - metabolism; Ribonucleases - metabolism; RNA; RNA Polymerase III - metabolism; RNA, Small Untranslated - biosynthesis; RNA, Small Untranslated - chemistry; RNA, Small Untranslated - metabolism; Templates, Genetic; Transcription, Genetic; Transfection

Synthetic RNA formulations and viral vectors are the two main approaches for delivering small therapeutic RNA to human cells. Here we report findings supporting an alternative strategy in which an endogenous human RNA polymerase (RNAP) is harnessed to make RNA hairpin-containing small RNA from synth...