miR-182-5p improves the viability, mitosis, migration, and invasion ability of human gastric cancer cells by down-regulating RAB27A

• Published in: Bioscience reports Vol. 37; no. 3
• Main Authors: Li, Yuling, Chen, Shudong, Shan, Zhengfei, Bi, Liyan, Yu, Shengqiang, Li, Yongwei, Xu, Sen
• Format: Journal Article
• Language: English
• Published: England Portland Press Ltd 30.06.2017
• Subjects: Cell Line, Tumor; Cell Movement; Cell Survival; Down-Regulation; Gene Expression Regulation, Neoplastic; Humans; MicroRNAs - genetics; Mitosis; Neoplasm Invasiveness - genetics; Neoplasm Invasiveness - pathology; rab27 GTP-Binding Proteins - genetics; Stomach Neoplasms - genetics; Stomach Neoplasms - pathology; gastric cancer; RAB27A; miR-182-5p
• DOI: 10.1042/BSR20170136
• ISSN: 0144-8463; 1573-4935

We investigated the effect of miR-182-5p on the viability, proliferation, invasion, and migration ability of human gastric cells by regulating the expression of RAB27A. Real-time PCR assay was used to detect the expression of miR-182-5 and RAB27A in human gastric carcinoma tissues, para-carcinoma tissues, and different cell lines. Western blotting was also used to determine the RAB27A expression in both tissues and cell lines. We chose the HGC-27 cell line as experiment subject as it demonstrated the highest miR-182-5p level. HGC-27 cells were transfected with different vectors and the cell viability, mitosis, invasion, and migration ability were measured through MTT assay, flow cytometry (FCM) analysis, Transwell assay, and wound healing assay. In comparison with the normal tissues, miR-182-5p is expressed at a higher level in gastric cancer (GC) tissues, while RAB27A is expressed at a lower level in cancerous tissues. The down-regulation of miR-182-5p and up-regulation of RAB27A can significantly decrease the viability, migration, invasion, and mitosis of HGC-27 cells. The target relationship between miR-182-5p and RAb27A was confirmed through a dual-luciferase reporter gene assay and Western blot assay. miR-182-5p enhances the viability, mitosis, migration, and invasion of human GC cells by down-regulating RAB27A.