Regulation of the Higher-Order Structure of Chromatin by Histones H1 and H5

• Published in: The Journal of cell biology Vol. 90; no. 2; pp. 279 - 288
• Main Authors: Allan, James, Cowling, Graham J., Harborne, Nerina, Cattini, Peter, Craigie, Robert, Gould, Hannah
• Format: Journal Article
• Language: English
• Published: United States Rockefeller University Press 01.08.1981; The Rockefeller University Press
• Subjects: Animals; Centrifugation, Density Gradient; Chickens; Chromatin; DNA; DNA - metabolism; Electrophoresis; Erythrocytes; Gels; Histones; Histones - physiology; Hot Temperature; Micrococcal Nuclease - metabolism; Microscopy, Electron; Molecules; Nucleic Acid Denaturation; Nucleosomes; Osmolar Concentration; Protein Conformation; Ratios

Chicken erythrocyte chromatins containing a single species of linker histone, H1 or H5, have been prepared, using reassembly techniques developed previously. The reconstituted complexes possess the conformation of native chicken erythrocyte chromatin, as judged by chemical and structural criteria; s...