Regulation of u‐PA gene expression in human prostate cancer

• Published in: International journal of cancer Vol. 94; no. 3; pp. 390 - 395
• Main Authors: Evans, Christopher P., Stapp, Eschelle C., Dall'Era, Marc A., Juarez, Jose, Yang, Joy C.
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 01.11.2001; Wiley-Liss
• Subjects: androgen; Biological and medical sciences; Blotting, Northern; Blotting, Western; Cell Division; Chloramphenicol O-Acetyltransferase - metabolism; Coloring Agents - pharmacology; Down-Regulation; Flutamide - pharmacology; Gene Expression Regulation, Neoplastic; Genes, Reporter; Humans; Male; MAP Kinase Signaling System; Medical sciences; Mutation; Nephrology. Urinary tract diseases; Promoter Regions, Genetic; prostate cancer; Prostatic Neoplasms - genetics; Prostatic Neoplasms - metabolism; Recombinant Fusion Proteins - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA - metabolism; Signal Transduction; Tetrazolium Salts - pharmacology; Thiazoles - pharmacology; Time Factors; Transcription, Genetic; transcriptional regulation; Transfection; Tumor Cells, Cultured; Tumors of the urinary system; Urinary tract. Prostate gland; urokinase; Urokinase-Type Plasminogen Activator - biosynthesis; Urokinase-Type Plasminogen Activator - genetics; Human; u-Plasminogen activator; Urinary system disease; Carcinoma; Prostate disease; Serine endopeptidases; Androgen; Enzyme; Transcription promoter; Malignant tumor; Gene expression; Peptidases; Signal transduction; Regulation(control); Established cell line; Genetics; Hydrolases; Sex steroid hormone; Male genital diseases; Prostate; Transcription factor AP1

u‐PA contributes to CaP progression, especially in the metastatic androgen‐insensitive state. In vitro, u‐PA is expressed by androgen‐insensitive, but not androgen‐sensitive, CaP cell lines. We hypothesized that in androgen‐sensitive CaP an activated ARE represses u‐PA expression but in androgen‐ins...