A highly efficient method for enriching TALEN or CRISPR/Cas9-edited mutant cells

In recent years,genome editing with site-specific nucleases,such as ZFN（zinc finger nuclease）,TALEN（transcription activatorlike effector nucleases）,and CRISPR/Cas9（the type II bacterial clustered,regularly interspaced,short palindromic repeats-associated protein 9）,has gained popularity for use in c...

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Published inJournal of genetics and genomics Vol. 43; no. 12; pp. 705 - 708
Main Authors Yasuda, Hideyo, Kim, Eunsu, Reza, Abu Musa Md Talimur, Kim, Jin-Hoi
Format Journal Article
LanguageEnglish
Published China Elsevier Ltd 20.12.2016
Subjects
Cell Separation - methods
CRISPR-Cas Systems - genetics
Gene Editing
HCT116 Cells
Humans
Transcription Activator-Like Effector Nucleases - metabolism
人才
位点特异性
核酸酶
相关蛋白
突变
细胞系
编辑
高效方法
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Summary:In recent years,genome editing with site-specific nucleases,such as ZFN（zinc finger nuclease）,TALEN（transcription activatorlike effector nucleases）,and CRISPR/Cas9（the type II bacterial clustered,regularly interspaced,short palindromic repeats-associated protein 9）,has gained popularity for use in cell lines,animals,and plants（Urnov et al.,2010;Miller et al.,2011;Cong et al.
Bibliography:In recent years,genome editing with site-specific nucleases,such as ZFN（zinc finger nuclease）,TALEN（transcription activatorlike effector nucleases）,and CRISPR/Cas9（the type II bacterial clustered,regularly interspaced,short palindromic repeats-associated protein 9）,has gained popularity for use in cell lines,animals,and plants（Urnov et al.,2010;Miller et al.,2011;Cong et al.
11-5450/R
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ISSN:1673-8527
DOI:10.1016/j.jgg.2016.10.006