Integrin Dependent RhoB Activation Assay Using Leukocytes
To be able to migrate, leukocyte needs to re-use its adhesion molecules to move forward. These adhesion molecules are called integrins and are intracellularly transported via endocytosis and exocytosis in order to translocate to a new site on the cell membrane. The intracellular transportation is re...
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| Published in | Bio-protocol Vol. 8; no. 23; p. e3105 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Bio-Protocol
05.12.2018
Bio-protocol LLC |
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| Online Access | Get full text |
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| Abstract | To be able to migrate, leukocyte needs to re-use its adhesion molecules to move forward. These adhesion molecules are called integrins and are intracellularly transported via endocytosis and exocytosis in order to translocate to a new site on the cell membrane. The intracellular transportation is regulated by different small GTPases including RhoB. Here we describe an activation assay of RhoB in leukocytes migrating on ICAM-1Fc coated dishes using commercially available Rhoteikin coated agarose beads. Although this is a specific protocol for LFA-1 induced RhoB activation, it can also be used for RhoB activation induced by other soluble and insoluble factors. |
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| AbstractList | To be able to migrate, leukocyte needs to re-use its adhesion molecules to move forward. These adhesion molecules are called integrins and are intracellularly transported via endocytosis and exocytosis in order to translocate to a new site on the cell membrane. The intracellular transportation is regulated by different small GTPases including RhoB. Here we describe an activation assay of RhoB in leukocytes migrating on ICAM-1Fc coated dishes using commercially available Rhoteikin coated agarose beads. Although this is a specific protocol for LFA-1 induced RhoB activation, it can also be used for RhoB activation induced by other soluble and insoluble factors. |
| Author | Lehtonen, Janne Samuelsson, Malin Svensson, Lena Potrzebowska, Katarzyna |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/34532550$$D View this record in MEDLINE/PubMed https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-72536$$DView record from Swedish Publication Index |
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| Copyright | Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC. Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC. 2018 |
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| Keywords | Leukocytes Pull-down LFA-1 Rhoteikin Small GTPases RhoB |
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| Title | Integrin Dependent RhoB Activation Assay Using Leukocytes |
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