Genomic survey and gene expression analysis of the VDAC gene family in rice

• Published in: Genetics and molecular research Vol. 14; no. 4; pp. 15683 - 15696
• Main Authors: Xu, X, Tan, Y P, Cheng, G, Liu, X Q, Xia, C J, Luo, F Y, Wang, C T
• Format: Journal Article
• Language: English
• Published: Brazil 01.01.2015
• Subjects: Chromosome Mapping; Cluster Analysis; Computational Biology - methods; Gene Duplication; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene Order; Gene Regulatory Networks; Genetic Loci; Genome, Plant; Genomics; Multigene Family; Oryza - classification; Oryza - genetics; Oryza - metabolism; Oryza sativa; Phylogeny; Voltage-Dependent Anion Channels - genetics; Voltage-Dependent Anion Channels - metabolism
• DOI: 10.4238/2015.December.1.20
• ISSN: 1676-5680

The voltage-dependent anion channel (VDAC), also known as a mitochondrial porin, plays an important role in the regulation of metabolic and energetic functions of mitochondria, as well as in mitochondria-mediated apoptosis. Cytoplasmic male sterility (CMS) is of major economic importance for commercial hybrid production and a research model for the interaction be-tween nuclear and cytoplasmic genomes. Recent research has revealed that CMS is associated with programmed cell death. Here, we used the Honglian (HL)-CMS line of rice (Oryza sativa) as material to investigate the association of O. sativa VDAC (OsVDAC) expression to CMS. Eight VDACs were extracted from rice in this study. Bioinformatic analysis of the rice VDACs was conducted at the DNA, cDNA, and protein level. Expression patterns of OsVDACs were analyzed in different organs and during different stages of pollen development using sterile line YuetaiA (YTA), and its maintainer line YuetaiB (YTB). Differential expression of OsVDACs between YTA and YTB was observed, suggesting that VDACs may be involved in the formation of HL-CMS.