Elucidation of tropane alkaloid biosynthesis in Erythroxylum coca using a microbial pathway discovery platform

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 119; no. 49; p. e2215372119
• Main Authors: Chavez, Benjamin G, Srinivasan, Prashanth, Glockzin, Kayla, Kim, Neill, Montero Estrada, Olga, Jirschitzka, Jan, Rowden, Gage, Shao, Jonathan, Meinhardt, Lyndel, Smolke, Christina D, D'Auria, John C
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 06.12.2022
• Subjects: Alkaloids; Amine Oxidase (Copper-Containing); Amines; Angiosperms; Biological Sciences; Biosynthesis; Cluster analysis; Clustering; Coca; Cocaine; Cytochrome; Cytochrome P450; Cytochromes P450; Erythroxylaceae; Erythroxylum coca; Flavin; Metabolites; Methyltransferase; Microorganisms; Polyamines; Reductases; Saccharomyces cerevisiae; Solanaceae; Solanaceae - genetics; Spermidine; Spermidine synthase; Transcriptomes; Tropane alkaloids; Tropanes; Yeast; Yeasts; N-methylspermidine; pathway discovery; Erythroxylaceae; tropane alkaloid; convergent evolution

Tropane alkaloids (TAs) are heterocyclic nitrogenous metabolites found across seven orders of angiosperms, including Malpighiales (Erythroxylaceae) and Solanales (Solanaceae). Despite the well-established euphorigenic properties of Erythroxylaceae TAs like cocaine, their biosynthetic pathway remains...