Comparison of detection methods for citrus tristeza virus in field trees during months of nonoptimal titer

• Published in: Plant disease Vol. 81; no. 5; p. 525
• Main Authors: Mathews, D.M. (University of California, Riverside.), Riley, K, Dodds, J.A
• Format: Journal Article
• Language: English
• Published: United States 01.05.1997
• Subjects: CITRUS SINENSIS; CLOSTEROVIRUS TRISTEZA DEL CITRUS; CLOSTEROVIRUS TRISTEZA DU CITRUS; DIAGNOSTIC; DIAGNOSTICO; ELISA; ENFERMEDADES DE LAS PLANTAS; MALADIE DES PLANTES; PCR; TEST ELISA

Enzyme-linked immunosorbent assay (ELISA) can reliably detect citrus tristeza virus (CTV) in samples collected during approximately 6 months of a typical year. Two reverse transcriptase polymerase chain reaction (RT-PCR) methods (total nucleic acid extract and immunocapture based) were evaluated and...