Host-Virus Interaction in Ribonucleic Acid Bacteriophage-Infected Escherichia coli I. Location of “Late” MS2-Specific Ribonucleic Acid Synthesis

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Published inJournal of Virology Vol. 4; no. 4; pp. 364 - 371
Main Authors Haywood, A M, Cramer, J H, Shoemaker, N L
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.10.1969
Subjects
Bacterial Viruses
Bacteriophages - metabolism
Bile Acids and Salts
Binding Sites
Carbon Isotopes
Cell Membrane - analysis
Centrifugation
Dactinomycin - pharmacology
Edetic Acid
Escherichia coli - analysis
Escherichia coli - drug effects
Magnesium
Ribosomes - analysis
RNA, Viral - biosynthesis
Time Factors
Tritium
Ultracentrifugation
Uracil
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Abstract Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0022-538X Online ISSN: 1098-5514 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JVI .asm.org, visit: JVI       
AbstractList When actinomycin-treated, MS2-infected Escherichia coli are labeled during a brief period later than 16 min after infection, the newly synthesized MS2 ribonucleic acid (RNA) appears first in the 30,000 × g sediment, probably bound to fragments of bacterial membranes, since the radioactivity can be released from the sediment with deoxycholate or urea. With longer labeling times, radioactivity also appears in the 30,000 × g supernatant fluid. While on the membrane, the RNA is organized into particles with sedimentation coefficients of 40, 32, and 27 S in the presence of low Mg 2+ . In the presence of high Mg + , MS2-specific RNA is found in polyribosomes. These data are interpreted to mean that MS2-specific RNA is synthesized and organized into larger structures on membrane. More than 8 min of labeling is required before radioactivity is found in the 81 S virion which appears in the supernatant fluid.
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When actinomycin-treated, MS2-infected Escherichia coli are labeled during a brief period later than 16 min after infection, the newly synthesized MS2 ribonucleic acid (RNA) appears first in the 30,000 x g sediment, probably bound to fragments of bacterial membranes, since the radioactivity can be released from the sediment with deoxycholate or urea. With longer labeling times, radioactivity also appears in the 30,000 x g supernatant fluid. While on the membrane, the RNA is organized into particles with sedimentation coefficients of 40, 32, and 27S in the presence of low Mg(2+). In the presence of high Mg(+), MS2-specific RNA is found in polyribosomes. These data are interpreted to mean that MS2-specific RNA is synthesized and organized into larger structures on membrane. More than 8 min of labeling is required before radioactivity is found in the 81S virion which appears in the supernatant fluid.
Author Jane Harris Cramer
Anne M. Haywood
Nadja Lancaster Shoemaker
AuthorAffiliation Department of Microbiology, Northwestern University Medical School, Chicago, Illinois 60611
Department of Microbiology, Yale University Medical School, New Haven, Connecticut 06510
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Present address: Division of Biology, California Institute of Technology, Pasadena, Calif. 91109.
Present address: Biology Department, Northwestern University, Evanston, Ill. 60201.
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When actinomycin-treated, MS2-infected Escherichia coli are labeled during a brief period later than 16 min after infection, the newly synthesized MS2...
When actinomycin-treated, MS2-infected Escherichia coli are labeled during a brief period later than 16 min after infection, the newly synthesized MS2...
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SubjectTerms Bacterial Viruses
Bacteriophages - metabolism
Bile Acids and Salts
Binding Sites
Carbon Isotopes
Cell Membrane - analysis
Centrifugation
Dactinomycin - pharmacology
Edetic Acid
Escherichia coli - analysis
Escherichia coli - drug effects
Magnesium
Ribosomes - analysis
RNA, Viral - biosynthesis
Time Factors
Tritium
Ultracentrifugation
Uracil
Title Host-Virus Interaction in Ribonucleic Acid Bacteriophage-Infected Escherichia coli I. Location of “Late” MS2-Specific Ribonucleic Acid Synthesis
URI http://jvi.asm.org/content/4/4/364.abstract
https://www.ncbi.nlm.nih.gov/pubmed/4980849
https://search.proquest.com/docview/84270729
https://pubmed.ncbi.nlm.nih.gov/PMC375883
Volume 4
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