Host-Virus Interaction in Ribonucleic Acid Bacteriophage-Infected Escherichia coli I. Location of “Late” MS2-Specific Ribonucleic Acid Synthesis
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Published in | Journal of Virology Vol. 4; no. 4; pp. 364 - 371 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
American Society for Microbiology
01.10.1969
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AbstractList | When actinomycin-treated, MS2-infected
Escherichia coli
are labeled during a brief period later than 16 min after infection, the newly synthesized MS2 ribonucleic acid (RNA) appears first in the 30,000 ×
g
sediment, probably bound to fragments of bacterial membranes, since the radioactivity can be released from the sediment with deoxycholate or urea. With longer labeling times, radioactivity also appears in the 30,000 ×
g
supernatant fluid. While on the membrane, the RNA is organized into particles with sedimentation coefficients of 40, 32, and 27
S
in the presence of low Mg
2+
. In the presence of high Mg
+
, MS2-specific RNA is found in polyribosomes. These data are interpreted to mean that MS2-specific RNA is synthesized and organized into larger structures on membrane. More than 8 min of labeling is required before radioactivity is found in the 81
S
virion which appears in the supernatant fluid. Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0022-538X Online ISSN: 1098-5514 Copyright © 2014 by the American Society for Microbiology. For an alternate route to JVI .asm.org, visit: JVI When actinomycin-treated, MS2-infected Escherichia coli are labeled during a brief period later than 16 min after infection, the newly synthesized MS2 ribonucleic acid (RNA) appears first in the 30,000 x g sediment, probably bound to fragments of bacterial membranes, since the radioactivity can be released from the sediment with deoxycholate or urea. With longer labeling times, radioactivity also appears in the 30,000 x g supernatant fluid. While on the membrane, the RNA is organized into particles with sedimentation coefficients of 40, 32, and 27S in the presence of low Mg(2+). In the presence of high Mg(+), MS2-specific RNA is found in polyribosomes. These data are interpreted to mean that MS2-specific RNA is synthesized and organized into larger structures on membrane. More than 8 min of labeling is required before radioactivity is found in the 81S virion which appears in the supernatant fluid. |
Author | Jane Harris Cramer Anne M. Haywood Nadja Lancaster Shoemaker |
AuthorAffiliation | Department of Microbiology, Northwestern University Medical School, Chicago, Illinois 60611 Department of Microbiology, Yale University Medical School, New Haven, Connecticut 06510 |
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Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present address: Division of Biology, California Institute of Technology, Pasadena, Calif. 91109. Present address: Biology Department, Northwestern University, Evanston, Ill. 60201. |
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Mendeley... When actinomycin-treated, MS2-infected Escherichia coli are labeled during a brief period later than 16 min after infection, the newly synthesized MS2... When actinomycin-treated, MS2-infected Escherichia coli are labeled during a brief period later than 16 min after infection, the newly synthesized MS2... |
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StartPage | 364 |
SubjectTerms | Bacterial Viruses Bacteriophages - metabolism Bile Acids and Salts Binding Sites Carbon Isotopes Cell Membrane - analysis Centrifugation Dactinomycin - pharmacology Edetic Acid Escherichia coli - analysis Escherichia coli - drug effects Magnesium Ribosomes - analysis RNA, Viral - biosynthesis Time Factors Tritium Ultracentrifugation Uracil |
Title | Host-Virus Interaction in Ribonucleic Acid Bacteriophage-Infected Escherichia coli I. Location of “Late” MS2-Specific Ribonucleic Acid Synthesis |
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