Amedibacterium intestinale gen. nov., sp. nov., isolated from human faeces, and reclassification of Eubacterium dolichum Moore et al . 1976 (Approved Lists 1980) as Amedibacillus dolichus gen. nov., comb. nov

• Published in: International journal of systematic and evolutionary microbiology Vol. 70; no. 6; pp. 3656 - 3664
• Main Authors: Ikeyama, Nao, Toyoda, Atsushi, Morohoshi, Sho, Kunihiro, Tadao, Murakami, Takumi, Mori, Hiroshi, Iino, Takao, Ohkuma, Moriya, Sakamoto, Mitsuo
• Format: Journal Article
• Language: English
• Published: England 01.06.2020
• Subjects: Absiella; hsp60; human faeces; Amedibacillus; Amedibacterium
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.004215

Four strains (9CBEGH2 , 9BBH35, 6BBH38 and 6EGH11) of Gram-stain-positive, obligately anaerobic, rod-shaped bacteria were isolated from faecal samples from healthy Japanese humans. The results of 16S rRNA gene sequence analysis indicated that the four strains represented members of the family and formed a monophyletic cluster with ' ' strain N6H1-5 (99.4% sequence similarity) and sp. Marseille-P5640 (99.3 %). JCM 10413 (94.2 %) and ATCC 25548 (93.7 %) were located near this monophyletic cluster. The isolates, 9CBEGH2 , ' ' JCM 30884 and sp. Marseille-P5640 shared 98.7-99.1% average nucleotide identity (ANI) with each other. Moreover, the DNA-DNA hybridization (DDH) values among three strains were 88.4-90.6%, indicating that these strains represent the same species. Strain 9CBEGH2 showed 21.5-24.1 % DDH values with other related taxa. In addition, the ANI values between strain 9CBEGH2 and other related taxa ranged from 71.2 % to 73.5 %, indicating that this strain should be considered as representing a novel species on the basis of whole-genome relatedness. Therefore, we formally propose a novel name for ' ' strains identified because the name ' ' has been effectively, but not validly, published since 2017. On the basis of the collected data, strain 9CBEGH2 represents a novel species of a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is 9CBEGH2 (=JCM 33778 =DSM 110575 ).