Rumen Fluid from Slaughtered Animals: A Standardized Procedure for Sampling, Storage and Use in Digestibility Trials

• Published in: Methods and protocols Vol. 5; no. 4; p. 59
• Main Authors: Fortina, Riccardo, Glorio Patrucco, Sara, Barbera, Salvatore, Tassone, Sonia
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 13.07.2022; MDPI
• Subjects: Abattoirs; Animals; Digestibility; Feeds; in vitro digestibility; Inoculum; Laboratories; Microorganisms; Protocol; Rumen; rumen fluid collection; rumen fluid storage; Sampling; slaughtered ruminants; United States > US; Macedon

Digestibility trials need a viable rumen fluid as inoculum to degrade feeds. The variability of rumen fluid depends on the animal’s diet, while its viability is greatly influenced by the sampling and handling procedures. In this article, we present a replicable protocol for sampling the rumen fluid...