Genetic characterization of the lobster pathogen Aerococcus viridans var. homari by 16S rRNA gene sequence and RAPD

• Published in: Diseases of aquatic organisms Vol. 63; no. 2-3; pp. 237 - 246
• Main Authors: GREENWOOD, Spencer J, KEITH, Ian R, DESPRES, Béatrice M, CAWTHORN, Richard J
• Format: Journal Article
• Language: English
• Published: Oldendorf Inter-Research 28.02.2005
• Subjects: Animals; Atlantic Ocean; Bacteriology; Base Sequence; Biological and medical sciences; Cluster Analysis; Crustacea; DNA Primers; Fundamental and applied biological sciences. Psychology; Genetic Variation; Geography; Invertebrates; Marine; Microbiology; Molecular Sequence Data; Nephropidae - microbiology; Pathology; Phenotype; Phylogeny; Random Amplified Polymorphic DNA Technique; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Streptococcaceae - classification; Streptococcaceae - genetics; Systematics; Atlantic Ocean; Aerococcus viridans; 16S rRNA; Genetic variability; RAPD; Zoopathogen; Phylogeny; Random amplified polymorphic DNA marker; Macrura; Crustacea; Marine environment; Streptococcaceae; Homarus americanus; Edible crustacean; Aerococcus viridans var. homari; Lobster; Gene; Arthropoda; 16S-RNA; Micrococcales; Bacteria; Decapoda; Gaffkemia; Invertebrata

A combination of 16S rRNA sequencing and random amplified polymorphic DNA (RAPD) analysis was used to evaluate the genetic diversity within Aerococcus viridans var. homari, the causative agent of gaffkemia in lobsters. A collection of 7 A. viridans var. homari strains and 2 avirulent A. viridans-lik...