Genetic characterization of the lobster pathogen Aerococcus viridans var. homari by 16S rRNA gene sequence and RAPD
A combination of 16S rRNA sequencing and random amplified polymorphic DNA (RAPD) analysis was used to evaluate the genetic diversity within Aerococcus viridans var. homari, the causative agent of gaffkemia in lobsters. A collection of 7 A. viridans var. homari strains and 2 avirulent A. viridans-lik...
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Published in | Diseases of aquatic organisms Vol. 63; no. 2-3; pp. 237 - 246 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Oldendorf
Inter-Research
28.02.2005
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Abstract | A combination of 16S rRNA sequencing and random amplified polymorphic DNA (RAPD) analysis was used to evaluate the genetic diversity within Aerococcus viridans var. homari, the causative agent of gaffkemia in lobsters. A collection of 7 A. viridans var. homari strains and 2 avirulent A. viridans-like cocci isolated from homarid lobsters harvested from different regions on the Atlantic Coast of North America were analyzed. The isolates are separated geographically and temporally between the years 1947 and 2000. Sequencing of 16S rRNA genes confirmed the inclusion of all 9 isolates in the monophyletic A. viridans clade (99.8 to 100% similarity). RAPD analysis revealed that the 9 A. viridans var. homari isolates could be separated into 2 distinct subtypes. Subtype 1 included the 7 pathogenic lobster isolates and constituted a homogeneous group regardless of their geographical, temporal or virulence differences. Subtype 2 contained the 2 avirulent A. viridans-like cocci that had distinct RAPD patterns and clustered separately with the non-marine A. viridans. RAPD analysis represented a useful method for determining molecular subtyping for the intraspecific classification and epidemiological investigations of A. viridans var. homari. |
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AbstractList | A combination of 16S rRNA sequencing and random amplified polymorphic DNA (RAPD) analysis was used to evaluate the genetic diversity within Aerococcus viridans var. homari, the causative agent of gaffkemia in lobsters. A collection of 7 A. viridans var. homari strains and 2 avirulent A. viridans-like cocci isolated from homarid lobsters harvested from different regions on the Atlantic Coast of North America were analyzed. The isolates are separated geographically and temporally between the years 1947 and 2000. Sequencing of 16S rRNA genes confirmed the inclusion of all 9 isolates in the monophyletic A. viridans clade (99.8 to 100% similarity). RAPD analysis revealed that the 9 A. viridans var. homari isolates could be separated into 2 distinct subtypes. Subtype 1 included the 7 pathogenic lobster isolates and constituted a homogeneous group regardless of their geographical, temporal or virulence differences. Subtype 2 contained the 2 avirulent A. viridans-like cocci that had distinct RAPD patterns and clustered separately with the non-marine A. viridans. RAPD analysis represented a useful method for determining molecular subtyping for the intraspecific classification and epidemiological investigations of A. viridans var. homari. |
Author | KEITH, Ian R GREENWOOD, Spencer J CAWTHORN, Richard J DESPRES, Béatrice M |
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Keywords | Aerococcus viridans 16S rRNA Genetic variability RAPD Zoopathogen Phylogeny Random amplified polymorphic DNA marker Macrura Crustacea Marine environment Streptococcaceae Homarus americanus Edible crustacean Aerococcus viridans var. homari Lobster Gene Arthropoda 16S-RNA Micrococcales Bacteria Decapoda Gaffkemia Invertebrata |
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SubjectTerms | Animals Atlantic Ocean Bacteriology Base Sequence Biological and medical sciences Cluster Analysis Crustacea DNA Primers Fundamental and applied biological sciences. Psychology Genetic Variation Geography Invertebrates Marine Microbiology Molecular Sequence Data Nephropidae - microbiology Pathology Phenotype Phylogeny Random Amplified Polymorphic DNA Technique RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Streptococcaceae - classification Streptococcaceae - genetics Systematics |
Title | Genetic characterization of the lobster pathogen Aerococcus viridans var. homari by 16S rRNA gene sequence and RAPD |
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