Development and Validation of an Image Analysis System for the Measurement of Cell Proliferation in Mammary Glands of Rats

• Published in: Toxicologic pathology Vol. 47; no. 5; p. 634
• Main Authors: Lindauer, Klaus, Bartels, Thomas, Scherer, Petra, Kabiri, Mostafa
• Format: Journal Article
• Language: English
• Published: United States 01.07.2019
• Subjects: Animals; Cell Proliferation - drug effects; Drug Evaluation, Preclinical - methods; Drug Evaluation, Preclinical - standards; Female; Image Processing, Computer-Assisted - methods; Image Processing, Computer-Assisted - standards; Insulin - analogs & derivatives; Insulin - toxicity; Mammary Glands, Animal - diagnostic imaging; Mammary Glands, Animal - drug effects; Mammary Glands, Animal - pathology; Photomicrography - methods; Photomicrography - standards; Rats, Sprague-Dawley; Reproducibility of Results; Software; Toxicity Tests - methods; Toxicity Tests - standards; mammary glands; cell proliferation; insulin analogs; rats; Ki67; image analysis; validation

Reliable detection and measurement of cell proliferation are essential in the preclinical assessment of carcinogenic risk of therapeutics. In this context, the assessment of mitogenic potential on mammary glands is crucial in the preclinical safety evaluation of novel insulins. The existing manual c...