Interaction of insulin-like growth factor I with porcine thyroid cells cultured in monolayer

• Published in: Endocrinology (Philadelphia) Vol. 121; no. 2; p. 749
• Main Authors: Saji, M, Tsushima, T, Isozaki, O, Murakami, H, Ohba, Y, Sato, K, Arai, M, Mariko, A, Shizume, K
• Format: Journal Article
• Language: English
• Published: United States 01.08.1987
• Subjects: Animals; Binding, Competitive; Cell Division; Cells, Cultured; Cyclic AMP - biosynthesis; DNA - biosynthesis; Insulin - analogs & derivatives; Insulin - metabolism; Insulin - pharmacology; Insulin-Like Growth Factor I - metabolism; Insulin-Like Growth Factor I - pharmacology; Insulin-Like Growth Factor II - metabolism; Insulin-Like Growth Factor II - pharmacology; Iodine Radioisotopes; Kinetics; Receptor, Insulin - metabolism; Receptors, Somatomedin; Somatomedins - metabolism; Swine; Thyroid Gland - drug effects; Thyroid Gland - metabolism

The interaction of insulin-like growth factor I (IGF-I) with porcine thyroid cells cultured in monolayer was studied. Specific binding of [125I]iodo-IGF-I to thyroid cells was a reversible process dependent on the time and temperature of incubation. A steady state was achieved in 18 h at 4 C and ave...