Sequence-specific endonuclease Bam HI. Effect of hydrophobic reagents on sequence recognition and catalysis

• Published in: The Journal of biological chemistry Vol. 255; no. 14; pp. 6521 - 6524
• Main Authors: George, J, Blakesley, R W, Chirikjian, J G
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 25.07.1980
• Subjects: Bacteriophage phi X 174; Deoxyribonuclease BamHI; DNA Restriction Enzymes - metabolism; DNA, Viral; Glycerol - pharmacology; Kinetics; Substrate Specificity

The specificity of cleavage of Bam HI is altered in the presence of hydrophobic reagents, such as glycerol and M2SO. The enzyme with altered specificity, designated Bam HI.1, generated digestion patterns of various DNAs, which were distinct from those generated by Bam HI. Cleavage sites recognized i...