Heterodimerization among thyroid hormone receptor, retinoic acid receptor, retinoid X receptor, chicken ovalbumin upstream promoter transcription factor, and an endogenous liver protein

• Published in: Molecular endocrinology (Baltimore, Md.) Vol. 6; no. 9; pp. 1468 - 1478
• Main Authors: BERRODIN, T. J, MARKS, M. S, OZATO, K, LINNEY, E, LAZAR, M. A
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Endocrine Society 01.09.1992
• Subjects: Animals; Binding Sites; Biological and medical sciences; Carrier Proteins - metabolism; COUP Transcription Factor I; DNA - metabolism; DNA-Binding Proteins - metabolism; Fundamental and applied biological sciences. Psychology; Liver - chemistry; Molecular and cellular biology; Molecular genetics; Nuclear Proteins - metabolism; Protein Binding; Protein Conformation; Protein Multimerization; Receptors, Cell Surface - metabolism; Receptors, Retinoic Acid; Receptors, Thyroid Hormone - metabolism; Recombinant Fusion Proteins - metabolism; Regulatory Sequences, Nucleic Acid; Retinoid X Receptors; Transcription Factors - metabolism; Transcription. Transcription factor. Splicing. Rna processing; Rat; Liver; Rodentia; Molecular interaction; Retinoids; Response element; Vertebrata; Mammalia; Thyroid hormone; Regulatory sequence; Triiodothyronine; Transcription factor; Dimerization; Retinoic acid; Hormonal receptor
• DOI: 10.1210/me.6.9.1468
• ISSN: 0888-8809

Thyroid hormone receptor (TR) binds to DNA as a monomer, homodimer, and heterodimer with nuclear proteins. We have confirmed that the TR can heterodimerize with retinoid X receptors (RXRs)-alpha and -beta, and have found that another member of the nuclear receptor superfamily, chicken ovalbumin upstream promoter transcription factor (COUP-TF), also formed heterodimers with the TR in the context of binding to a palindromic thyroid hormone-responsive element (TREp). The interaction between COUP-TF and the TR was confirmed using specific antibodies which supershifted the COUP-TF/TR DNA complexes. The complex between the TR and the major TR heterodimerization partner in liver was unaffected by antibodies to COUP-TF and RXR beta, but was supershifted by an anti-RXR alpha antibody, indicating that the liver protein is highly related to RXR alpha. Indeed, the TR/RXR and TR/liver protein heterodimers contact the same guanidine residues in TREp. The retinoic acid receptor (RAR) also heterodimerized with COUP-TF as well as with RXR alpha, RXR beta, and the TR heterodimerization partner in liver. In contrast to its ability to heterodimerize with the TR and RAR, we did not detect heterodimers between COUP-TF and either RXR alpha, RXR beta, or the liver nuclear protein in the context of binding to the TREp. These results show that the major TR heterodimerization partner in liver is highly related to RXR alpha, but that other nuclear receptors such as COUP-TF can heterodimerize with the TR and RAR, suggesting that selective protein-protein interactions may be involved in the tissue and target gene specificities of hormone action.