Structure and Genetic Analysis of the Arterivirus Nonstructural Protein 7α
Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from...
Saved in:
| Published in | Journal of Virology Vol. 85; no. 14; pp. 7449 - 7453 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Washington, DC
American Society for Microbiology
01.07.2011
|
| Series | Notes |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0022-538X 1098-5514 |
| DOI | 10.1128/JVI.00255-11 |
Cover
Loading…
| Abstract | Article Usage Stats
Services
JVI
Citing Articles
Google Scholar
PubMed
Related Content
Social Bookmarking
CiteULike
Delicious
Digg
Facebook
Google+
Mendeley
Reddit
StumbleUpon
Twitter
current issue
Spotlights in the Current Issue
JVI
About
JVI
Subscribers
Authors
Reviewers
Advertisers
Inquiries from the Press
Permissions & Commercial Reprints
ASM Journals Public Access Policy
JVI
RSS Feeds
1752 N Street N.W. • Washington DC 20036
202.737.3600 • 202.942.9355 fax • journals@asmusa.org
Print ISSN:
0022-538X
Online ISSN:
1098-5514
Copyright © 2014
by the
American Society for Microbiology.
For an alternate route to
JVI
.asm.org, visit:
JVI
|
|---|---|
| AbstractList | Article Usage Stats
Services
JVI
Citing Articles
Google Scholar
PubMed
Related Content
Social Bookmarking
CiteULike
Delicious
Digg
Facebook
Google+
Mendeley
Reddit
StumbleUpon
Twitter
current issue
Spotlights in the Current Issue
JVI
About
JVI
Subscribers
Authors
Reviewers
Advertisers
Inquiries from the Press
Permissions & Commercial Reprints
ASM Journals Public Access Policy
JVI
RSS Feeds
1752 N Street N.W. • Washington DC 20036
202.737.3600 • 202.942.9355 fax • journals@asmusa.org
Print ISSN:
0022-538X
Online ISSN:
1098-5514
Copyright © 2014
by the
American Society for Microbiology.
For an alternate route to
JVI
.asm.org, visit:
JVI
Arterivirus replicase polyproteins are cleaved into at least 13 mature nonstructural proteins (nsps), and in particular the nsp5-to-nsp8 region is subject to a complex processing cascade. The function of the largest subunit from this region, nsp7, which is further cleaved into nsp7 alpha and nsp7 beta , is unknown. Using nuclear magnetic resonance (NMR) spectroscopy, we determined the solution structure of nsp7 alpha of equine arteritis virus, revealing an interesting unique fold for this protein but thereby providing little clue to its possible functions. Nevertheless, structure-based reverse genetics studies established the importance of nsp7/nsp7 alpha for viral RNA synthesis, thus providing a basis for future studies. Arterivirus replicase polyproteins are cleaved into at least 13 mature nonstructural proteins (nsps), and in particular the nsp5-to-nsp8 region is subject to a complex processing cascade. The function of the largest subunit from this region, nsp7, which is further cleaved into nsp7α and nsp7β, is unknown. Using nuclear magnetic resonance (NMR) spectroscopy, we determined the solution structure of nsp7α of equine arteritis virus, revealing an interesting unique fold for this protein but thereby providing little clue to its possible functions. Nevertheless, structure-based reverse genetics studies established the importance of nsp7/nsp7α for viral RNA synthesis, thus providing a basis for future studies. |
| Author | Clara C. Posthuma Isabelle Imbert Geoff Kelly Paul A. Tucker Ioannis Manolaridis Jessika C. Zevenhoven-Dobbe Cyril Gaudin Bruno Canard Eric J. Snijder Maria R. Conte |
| Author_xml | – sequence: 1 givenname: Ioannis surname: MANOLARIDIS fullname: MANOLARIDIS, Ioannis organization: European Molecular Biology Laboratory, Hamburg Outstation, 22603 Hamburg, Germany – sequence: 2 givenname: Cyril surname: GAUDIN fullname: GAUDIN, Cyril organization: Randall Division of Cell and Molecular Biophysics, King's College London, New Hunt's House, Guy's Campus, London SE1 1UL, United Kingdom – sequence: 3 givenname: Clara C surname: POSTHUMA fullname: POSTHUMA, Clara C organization: Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, 2300 RC Leiden, Netherlands – sequence: 4 givenname: Jessika C surname: ZEVENHOVEN-DOBBE fullname: ZEVENHOVEN-DOBBE, Jessika C organization: Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, 2300 RC Leiden, Netherlands – sequence: 5 givenname: Isabelle surname: IMBERT fullname: IMBERT, Isabelle organization: Architecture et Fonction des Macromolecules Biologiques, CNRS and Universités d'Aix-Marseille I et II, UMR 6098, ESIL Case 925, Marseille, France – sequence: 6 givenname: Bruno surname: CANARD fullname: CANARD, Bruno organization: Architecture et Fonction des Macromolecules Biologiques, CNRS and Universités d'Aix-Marseille I et II, UMR 6098, ESIL Case 925, Marseille, France – sequence: 7 givenname: Geoff surname: KELLY fullname: KELLY, Geoff organization: Biomedical NMR Centre, National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom – sequence: 8 givenname: Paul A surname: TUCKER fullname: TUCKER, Paul A organization: European Molecular Biology Laboratory, Hamburg Outstation, 22603 Hamburg, Germany – sequence: 9 givenname: Maria R surname: CONTE fullname: CONTE, Maria R organization: Randall Division of Cell and Molecular Biophysics, King's College London, New Hunt's House, Guy's Campus, London SE1 1UL, United Kingdom – sequence: 10 givenname: Eric J surname: SNIJDER fullname: SNIJDER, Eric J organization: Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, 2300 RC Leiden, Netherlands |
| BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24332517$$DView record in Pascal Francis |
| BookMark | eNp1kd1qVDEUhYNU7LR65wMEQbzxtNn5Oyc3wlC0PxYVLOJdyMkknZQzSZvkjPSxfBGfyTOdUajg1Ya9vr32gnWA9mKKDqGXQI4AaHd88e38iBAqRAPwBM2AqK4RAvgemk1r2gjWfd9HB6XcEAKcS_4M7VMQEhTQGfr4tebR1jE7bOICn7roarB4Hs1wX0LByeO6dHieq8thHfJY8KcUy-7IDPhLTtWFiNtfP5-jp94Mxb3YzUN09eH91clZc_n59PxkftlY1qraCCd7xdzCeiV6Y4VgpOdWKtNSSQlI5X0_qQtgjHvrhPfOGtL7BSWKeWCH6N3W9nbsVxPoYp2C6NscVibf62SCfqzEsNTXaa0ZUClhY_BmZ5DT3ehK1atQrBsGE10ai-66DghXQk7k6x1pijWDzybaUP6-opwxKqCdOLrlbE6lZOe1DdXUkDYBwqCB6E1XeupKP3SlH2K8_efoj-9_8FdbfBmulz9CdtqUlb5ZB91NOtct54r9BvmIo-k |
| CitedBy_id | crossref_primary_10_1007_s11262_013_0957_4 crossref_primary_10_1038_s42004_021_00476_4 crossref_primary_10_1016_j_csbj_2022_06_059 crossref_primary_10_1128_JVI_02096_16 crossref_primary_10_3390_v13081549 crossref_primary_10_1016_j_virusres_2014_12_009 crossref_primary_10_1016_j_vetmic_2013_06_015 crossref_primary_10_3389_fmicb_2017_00853 crossref_primary_10_3389_fmicb_2018_00970 |
| Cites_doi | 10.1038/nprot.2007.406 10.1099/0022-1317-79-5-961 10.1016/0042-6822(92)90193-S 10.1021/bi052387u 10.1016/j.virusres.2010.07.016 10.1128/JVI.74.20.9586-9593.2000 10.1128/jvi.71.12.9313-9322.1997 10.1016/j.virusres.2010.07.030 10.1128/JVI.00261-09 10.1128/JVI.00564-07 10.1007/s12104-010-9258-1 10.1016/S0021-9258(19)77912-5 10.1128/JVI.74.11.5213-5223.2000 10.1016/j.virusres.2006.01.017 10.1099/vir.0.82269-0 10.1128/JVI.80.7.3428-3437.2006 10.1093/bioinformatics/btm404 10.1128/JVI.01358-06 10.1128/JVI.73.3.2027-2037.1999 10.1099/vir.0.81611-0 10.1002/j.1460-2075.1994.tb06587.x 10.1128/jvi.65.6.2910-2920.1991 10.1099/0022-1317-81-4-853 |
| ContentType | Journal Article |
| Copyright | 2015 INIST-CNRS Copyright © 2011, American Society for Microbiology 2011 American Society for Microbiology |
| Copyright_xml | – notice: 2015 INIST-CNRS – notice: Copyright © 2011, American Society for Microbiology 2011 American Society for Microbiology |
| DBID | AAYXX CITATION IQODW 7U9 8FD FR3 H94 P64 RC3 5PM |
| DOI | 10.1128/JVI.00255-11 |
| DatabaseName | CrossRef Pascal-Francis Virology and AIDS Abstracts Technology Research Database Engineering Research Database AIDS and Cancer Research Abstracts Biotechnology and BioEngineering Abstracts Genetics Abstracts PubMed Central (Full Participant titles) |
| DatabaseTitle | CrossRef AIDS and Cancer Research Abstracts Genetics Abstracts Virology and AIDS Abstracts Engineering Research Database Technology Research Database Biotechnology and BioEngineering Abstracts |
| DatabaseTitleList | AIDS and Cancer Research Abstracts CrossRef |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 1098-5514 |
| EndPage | 7453 |
| ExternalDocumentID | PMC3126611 24332517 10_1128_JVI_00255_11 jvi_85_14_7449 |
| GroupedDBID | --- -~X 0R~ 18M 29L 2WC 39C 4.4 53G 5GY 5RE 5VS 85S AAFWJ AAGFI AAYXX ABPPZ ACGFO ACNCT ADBBV AENEX AGVNZ ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BTFSW CITATION CS3 DIK E3Z EBS EJD F5P FRP GX1 H13 HYE HZ~ IH2 KQ8 N9A O9- OK1 P2P RHI RNS RPM RSF TR2 UPT W2D W8F WH7 WOQ YQT ~02 ~KM .55 .GJ 3O- 41~ 6TJ AAYJJ ADXHL AFFNX AI. C1A D0S IQODW MVM OHT VH1 X7M Y6R ZGI ZXP 7U9 8FD FR3 H94 P64 RC3 5PM |
| ID | FETCH-LOGICAL-c379t-5e6b93edcf95bac5530b4c69a72620169ffbedcd1334fce5ffeca0bfd2093f13 |
| ISSN | 0022-538X |
| IngestDate | Thu Aug 21 18:45:10 EDT 2025 Fri Jul 11 03:21:19 EDT 2025 Mon Jul 21 09:13:49 EDT 2025 Tue Jul 01 01:02:06 EDT 2025 Thu Apr 24 22:51:50 EDT 2025 Wed May 18 15:26:05 EDT 2016 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 14 |
| Keywords | Virus Genetics Arteriviridae Arterivirus Nidovirales Protein |
| Language | English |
| License | CC BY 4.0 |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c379t-5e6b93edcf95bac5530b4c69a72620169ffbedcd1334fce5ffeca0bfd2093f13 |
| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 These authors contributed equally to this work. Present address: Institut Génomique Fonctionelle de Lyon, Ens de Lyon, Lyon Cedex 07, France. Present address: The Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, United Kingdom. |
| PMID | 21561912 |
| PQID | 888104956 |
| PQPubID | 23462 |
| PageCount | 5 |
| ParticipantIDs | proquest_miscellaneous_888104956 crossref_citationtrail_10_1128_JVI_00255_11 highwire_asm_jvi_85_14_7449 pascalfrancis_primary_24332517 pubmedcentral_primary_oai_pubmedcentral_nih_gov_3126611 crossref_primary_10_1128_JVI_00255_11 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2011-07-01 |
| PublicationDateYYYYMMDD | 2011-07-01 |
| PublicationDate_xml | – month: 07 year: 2011 text: 2011-07-01 day: 01 |
| PublicationDecade | 2010 |
| PublicationPlace | Washington, DC |
| PublicationPlace_xml | – name: Washington, DC – name: 1752 N St., N.W., Washington, DC |
| PublicationSeriesTitle | Notes |
| PublicationTitle | Journal of Virology |
| PublicationYear | 2011 |
| Publisher | American Society for Microbiology |
| Publisher_xml | – name: American Society for Microbiology |
| References | e_1_3_3_17_2 e_1_3_3_16_2 e_1_3_3_18_2 Snijder E. J. (e_1_3_3_19_2) 2006 e_1_3_3_13_2 e_1_3_3_14_2 e_1_3_3_11_2 e_1_3_3_10_2 Maclachlan N. J. (e_1_3_3_12_2) 2007 e_1_3_3_6_2 e_1_3_3_5_2 e_1_3_3_8_2 e_1_3_3_7_2 Potterton E. (e_1_3_3_15_2) 2002; 58 e_1_3_3_9_2 e_1_3_3_27_2 e_1_3_3_24_2 e_1_3_3_23_2 e_1_3_3_26_2 e_1_3_3_25_2 e_1_3_3_2_2 e_1_3_3_20_2 e_1_3_3_4_2 e_1_3_3_22_2 e_1_3_3_3_2 e_1_3_3_21_2 |
| References_xml | – start-page: 1337 volume-title: Fields virology year: 2006 ident: e_1_3_3_19_2 – ident: e_1_3_3_3_2 doi: 10.1038/nprot.2007.406 – volume: 58 start-page: 1955 year: 2002 ident: e_1_3_3_15_2 article-title: The CCP4 molecular-graphics project publication-title: Acta Crystallogr. – ident: e_1_3_3_18_2 doi: 10.1099/0022-1317-79-5-961 – ident: e_1_3_3_10_2 doi: 10.1016/0042-6822(92)90193-S – ident: e_1_3_3_4_2 doi: 10.1021/bi052387u – start-page: 325 year: 2007 ident: e_1_3_3_12_2 article-title: Arterivirus pathogenesis and immune response publication-title: Nidoviruses – ident: e_1_3_3_26_2 doi: 10.1016/j.virusres.2010.07.016 – ident: e_1_3_3_17_2 doi: 10.1128/JVI.74.20.9586-9593.2000 – ident: e_1_3_3_24_2 doi: 10.1128/jvi.71.12.9313-9322.1997 – ident: e_1_3_3_6_2 doi: 10.1016/j.virusres.2010.07.030 – ident: e_1_3_3_13_2 doi: 10.1128/JVI.00261-09 – ident: e_1_3_3_2_2 doi: 10.1128/JVI.00564-07 – ident: e_1_3_3_7_2 doi: 10.1007/s12104-010-9258-1 – ident: e_1_3_3_25_2 doi: 10.1016/S0021-9258(19)77912-5 – ident: e_1_3_3_23_2 doi: 10.1128/JVI.74.11.5213-5223.2000 – ident: e_1_3_3_8_2 doi: 10.1016/j.virusres.2006.01.017 – ident: e_1_3_3_21_2 doi: 10.1099/vir.0.82269-0 – ident: e_1_3_3_20_2 doi: 10.1128/JVI.80.7.3428-3437.2006 – ident: e_1_3_3_9_2 doi: 10.1093/bioinformatics/btm404 – ident: e_1_3_3_16_2 doi: 10.1128/JVI.01358-06 – ident: e_1_3_3_22_2 doi: 10.1128/JVI.73.3.2027-2037.1999 – ident: e_1_3_3_14_2 doi: 10.1099/vir.0.81611-0 – ident: e_1_3_3_11_2 doi: 10.1002/j.1460-2075.1994.tb06587.x – ident: e_1_3_3_5_2 doi: 10.1128/jvi.65.6.2910-2920.1991 – ident: e_1_3_3_27_2 doi: 10.1099/0022-1317-81-4-853 |
| SSID | ssj0014464 |
| Score | 2.080044 |
| Snippet | Article Usage Stats
Services
JVI
Citing Articles
Google Scholar
PubMed
Related Content
Social Bookmarking
CiteULike
Delicious
Digg
Facebook
Google+
Mendeley... Arterivirus replicase polyproteins are cleaved into at least 13 mature nonstructural proteins (nsps), and in particular the nsp5-to-nsp8 region is subject to a... |
| SourceID | pubmedcentral proquest pascalfrancis crossref highwire |
| SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 7449 |
| SubjectTerms | Biological and medical sciences Equine arteritis virus Fundamental and applied biological sciences. Psychology Genetics Microbiology Miscellaneous Structure and Assembly Virology |
| Title | Structure and Genetic Analysis of the Arterivirus Nonstructural Protein 7α |
| URI | http://jvi.asm.org/content/85/14/7449.abstract https://www.proquest.com/docview/888104956 https://pubmed.ncbi.nlm.nih.gov/PMC3126611 |
| Volume | 85 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3bjtMwELWqRUi8IK4iLKz8QJ-qlCZ2Ln6EsuwFgZAoqG-Wc7E2CyRo2660_AMfw4_wTczEsZvArrTwYlWxnTY94_GMM3OGkGfI7xHnLPJDXsY-j1PuKy64L1igwyIoI3DgMNriXXz4kR8vo-Vo9KMXtbRZZ9P8-6V5Jf-DKlwDXDFL9h-QdTeFC_AZ8IUWEIb2Whh_aMlf7SsAZJBG-tU-z0ibOIJRm9V5dbYBndZ0jLEt28Z7JGmo6kkynu-PXwZXGKqYCdc_e3-ravSHq8LQExw1WPbImeYHalMYXoL5xdk2fgOLAmM9wLYDZqvJfOoOrZFF6qSBxn_VZJk51cfw3M9uVLE9aE3sucQ2TwDU6dLsNEa9Insp2mh9_WtK9lg54z1tmnBDZ9rtzAk3vMJ_a_0QMxmOPx1NWxfJ79T3gFz7j03PhSK2TlCYSpgt29kSM8VvhOB1YEGMg6WLGELPmVvyeXwwm0cRps_73z20cCzrNAbdqhWsO20Kpgw8mmE8bs_AWdwhtzvA6QsjZnfJqKzvkZumVunFffLGCRsFYaOdsFErbLTRFISN9oSNDoSNdsJGk18_H5DF6_3F_NDvKnH4OUvE2o_KOBMMfqAWUaZyLDWV8TwWKsF6BrDetc6gtwgY4zovIwxFUrNMF-FMMB2wh2SnburyEaECzMV8VjANZjlnGVNFoJmIYPvnoRJF7pGJ_fNk3rHUY7GUL_IyoDwydqO_GXaWK8btWhykWn2Vp-eVTKGDSxQxj-wNoHG3CpHcLwoSj1CLlQTti6_UVF02m5VM0zSY4RmDR5IBhu4eSN8-7Kmrk5bGnQVoHAePr_kMu-TWdpk9ITuAX_kUDOJ1tteK6W8wc7gI |
| linkProvider | Geneva Foundation for Medical Education and Research |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Structure+and+Genetic+Analysis+of+the+Arterivirus+Nonstructural+Protein+7%CE%B1&rft.jtitle=Journal+of+virology&rft.au=Manolaridis%2C+Ioannis&rft.au=Gaudin%2C+Cyril&rft.au=Posthuma%2C+Clara+C.&rft.au=Zevenhoven-Dobbe%2C+Jessika+C.&rft.date=2011-07-01&rft.issn=0022-538X&rft.eissn=1098-5514&rft.volume=85&rft.issue=14&rft.spage=7449&rft.epage=7453&rft_id=info:doi/10.1128%2FJVI.00255-11&rft.externalDBID=n%2Fa&rft.externalDocID=10_1128_JVI_00255_11 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0022-538X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0022-538X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0022-538X&client=summon |