A noncommercial polymerase chain reaction–based method to approach one hundred percent recombinant clone selection efficiency

• Published in: Analytical biochemistry Vol. 382; no. 1; pp. 75 - 76
• Main Authors: Shareef, Mohammed M., Dancea, Horatiu C., Gross, Jessica L., Myers, Tamara T., Griggs, Wendy W., Ahmed, Mansoor M., Sheldon, David G.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2008
• Subjects: Base Sequence; Cell Line, Tumor; Cloning, Molecular - methods; DNA, Recombinant - genetics; DNA, Recombinant - isolation & purification; Genetic Vectors - genetics; Humans; Polymerase Chain Reaction - methods

Molecular cloning is an important procedure in molecular biology, but this is often a rate-limiting step and can be very time-consuming, possibly due to low ligation efficiency. Here, we describe a simple polymerase chain reaction (PCR)-based strategy to approach 100% selection efficiency. The post-...