Profiling of gene-dependent translational progress in cell-free protein synthesis by real-space imaging

• Published in: Analytical biochemistry Vol. 394; no. 2; pp. 275 - 280
• Main Authors: Mikamo-Satoh, Eriko, Takagi, Akihiko, Tanaka, Hiroyuki, Matsumoto, Takuya, Nishihara, Tsutomu, Kawai, Tomoji
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.11.2009
• Subjects: Atomic force microscopy; Cell-free protein synthesis; Cell-Free System - metabolism; Escherichia coli - genetics; Genes; Green Fluorescent Proteins - metabolism; Humans; Microscopy, Atomic Force; Peptide Chain Initiation, Translational; Peptide Chain Termination, Translational; Plasmids - genetics; Polyribosomes - metabolism; Polyribosomes - ultrastructure; Polysome profile; Protein Biosynthesis; Protein expression; Protein Modification, Translational; Retinoid X Receptors - metabolism; Retinoid X Receptors - ultrastructure; Ribosomes - metabolism; Ribosomes - ultrastructure; RNA, Messenger - metabolism; Time Factors; Transformation, Bacterial; Translational progress; Atomic force microscopy; Protein expression; Translational progress; Cell-free protein synthesis; Polysome profile

In general, gene-dependent translational progress affects the efficiency of protein expression. To evaluate the translational progress of protein synthesis, it is necessary to trace the time course of translation as well as the quantity of products. Here we present a new method for tracking translat...