Uncoupling of transcription and translation of Fanconi anemia (FANC) complex proteins during spermatogenesis
Male germ cell genome integrity is critical for spermatogenesis, fertility and normal development of the offspring. Several DNA repair pathways exist in male germ cells. One such important pathway is the Fanconi anemia (FANC) pathway. Unlike in somatic cells, expression profiles and the role of the...
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Published in | Spermatogenesis Vol. 5; no. 1; p. e979061 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
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United States
Taylor & Francis
2015
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Abstract | Male germ cell genome integrity is critical for spermatogenesis, fertility and normal development of the offspring. Several DNA repair pathways exist in male germ cells. One such important pathway is the Fanconi anemia (FANC) pathway. Unlike in somatic cells, expression profiles and the role of the FANC pathway in germ cells remain largely unknown. In this study, we undertook an extensive expression analyses at both mRNA and protein levels of key components of the FANC pathway during spermatogenesis in the mouse. Herein we show that Fanc mRNAs and proteins displayed developmental enrichment within particular male germ cell types. Spermatogonia and pre-leptotene spermatocytes contained the majority of the FANC components examined i.e. complex I members FANCB, FANCG and FANCM, complex II members FANCD2 and FANCI, and complex III member FANCJ. Leptotene, zygotene and early pachytene spermatocytes contained FANCB, FANCG, FANCM and FANCD2. With the exception of FANCL, all FANC proteins examined were not detected in round spermatids. Elongating and elongated spermatids contained FANCB, FANCG, FANCL and FANCJ. qPCR analysis on isolated spermatocytes and round spermatids showed that Fancg, Fancl, Fancm, Fancd2, Fanci and Fancj mRNAs were expressed in both of these germ cell types, indicating that some degree of translational repression of these FANC proteins occurs during the transition from meiosis to spermiogenesis. Taken together, our findings raise the possibility that the assembly of FANC protein complexes in each of the male germ cell type is unique and may be distinct from the proposed model in mitotic cells. |
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AbstractList | Male germ cell genome integrity is critical for spermatogenesis, fertility and normal development of the offspring. Several DNA repair pathways exist in male germ cells. One such important pathway is the Fanconi anemia (FANC) pathway. Unlike in somatic cells, expression profiles and the role of the FANC pathway in germ cells remain largely unknown. In this study, we undertook an extensive expression analyses at both mRNA and protein levels of key components of the FANC pathway during spermatogenesis in the mouse. Herein we show that Fanc mRNAs and proteins displayed developmental enrichment within particular male germ cell types. Spermatogonia and pre-leptotene spermatocytes contained the majority of the FANC components examined i.e. complex I members FANCB, FANCG and FANCM, complex II members FANCD2 and FANCI, and complex III member FANCJ. Leptotene, zygotene and early pachytene spermatocytes contained FANCB, FANCG, FANCM and FANCD2. With the exception of FANCL, all FANC proteins examined were not detected in round spermatids. Elongating and elongated spermatids contained FANCB, FANCG, FANCL and FANCJ. qPCR analysis on isolated spermatocytes and round spermatids showed that Fancg, Fancl, Fancm, Fancd2, Fanci and Fancj mRNAs were expressed in both of these germ cell types, indicating that some degree of translational repression of these FANC proteins occurs during the transition from meiosis to spermiogenesis. Taken together, our findings raise the possibility that the assembly of FANC protein complexes in each of the male germ cell type is unique and may be distinct from the proposed model in mitotic cells. |
Author | O'Donnell, Liza O'Connor, Anne E O'Bryan, Moira K Jamsai, Duangporn Lo, Jennifer Chi Yi |
Author_xml | – sequence: 1 givenname: Duangporn surname: Jamsai fullname: Jamsai, Duangporn email: Duangporn.Jamsai@monash.edu organization: The Department of Anatomy and Developmental Biology; School of Biomedical Sciences; Faculty of Medicine; Nursing and Health Sciences; Monash University – sequence: 2 givenname: Anne E surname: O'Connor fullname: O'Connor, Anne E organization: The Department of Anatomy and Developmental Biology; School of Biomedical Sciences; Faculty of Medicine; Nursing and Health Sciences; Monash University – sequence: 3 givenname: Liza surname: O'Donnell fullname: O'Donnell, Liza organization: MIMR-PHI Institute of Medical Research – sequence: 4 givenname: Jennifer Chi Yi surname: Lo fullname: Lo, Jennifer Chi Yi organization: The Department of Anatomy and Developmental Biology; School of Biomedical Sciences; Faculty of Medicine; Nursing and Health Sciences; Monash University – sequence: 5 givenname: Moira K surname: O'Bryan fullname: O'Bryan, Moira K organization: The Department of Anatomy and Developmental Biology; School of Biomedical Sciences; Faculty of Medicine; Nursing and Health Sciences; Monash University |
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Title | Uncoupling of transcription and translation of Fanconi anemia (FANC) complex proteins during spermatogenesis |
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