Expression and antigenic analysis of the recombinant TRP36 protein from Ehrlichia canis São Paulo strain for serologic tests
Abstract Ehrlichia canis is the main etiological agent of canine monocytic ehrlichiosis (CME), a globally canine infectious disease. In Brazil, CME is considered to be endemic, and its prevalence can reach 65% in some states. The diagnosis of ehrlichiosis is important for treatment and epidemiologic...
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| Published in | Revista brasileira de parasitologia veterinaria Vol. 29; no. 3; p. e005820 |
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| Language | English Portuguese |
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Colégio Brasileiro de Parasitologia Veterinária
01.01.2020
Colégio Brasileiro de Parasitologia Veterinaria |
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| Abstract | Abstract Ehrlichia canis is the main etiological agent of canine monocytic ehrlichiosis (CME), a globally canine infectious disease. In Brazil, CME is considered to be endemic, and its prevalence can reach 65% in some states. The diagnosis of ehrlichiosis is important for treatment and epidemiological purposes. The E. canis TRP36 (Tandem Repeat Protein) protein elicits the earliest acute-phase antibody response observed during the course of the disease. This study aimed to generate the recombinant TRP36 protein from E. canis São Paulo strain and to evaluate its potential as a tool for the serologic diagnosis of CME. The E. canis São Paulo isolate was cultivated in DH82 lineage cells, and its genomic DNA was obtained. The bacterial DNA fragment encoding the entire ORF of TRP36 was cloned into the pBAD/Thio-TOPO vector and transformed into Escherichia coli DH10B competent cells with the trp36-bearing plasmid for protein expression. To evaluate the protein antigenicity, 16 canine serum samples were previously tested (by PCR and the commercial SNAP®4Dx® serological test). The results were in accordance with the SNAP®4Dx® test. Experiments using this recombinant protein as an antigen, targeting the development of a serologic test based on ELISA methodology, are the next step to produce a reliable, affordable and useful diagnostic tool for CME in Brazil.
Resumo Ehrlichia canis é o principal agente etiológico da erliquiose monocítica canina (EMC), uma doença infecciosa canina globalmente dispersa. No Brasil, a EMC é considerada endêmica, e a infecção pode atingir 65% em cães em alguns estados. O diagnóstico de erliquiose é importante para fins de tratamento e epidemiológicos. A proteína TRP36 de E. canis leva a uma resposta humoral com produção de anticorpos em fase aguda, encontrada durante o curso da doença. O objetivo deste estudo foi obter a proteína TRP36 recombinante da amostra São Paulo de E. canis e avaliar seu potencial como ferramenta para o diagnóstico sorológico da CME. O isolado de E. canis São Paulo foi cultivado em células da linhagem DH82 e o DNA genômico foi obtido. O fragmento de DNA bacteriano que codifica toda a ORF de TRP36 foi clonado no vetor pBAD / Thio-TOPO e transformado em células competentes Escherichia coli DH10B, com o plasmídeo portador de trp36 para expressão de proteínas. Para avaliar a antigenicidade da proteína, 16 amostras de soro canino foram previamente analisadas (por PCR e teste sorológico comercial SNAP®4Dx®). Os resultados estavam de acordo com o teste SNAP®4Dx®. Os experimentos que utilizam essa proteína recombinante como antígeno, visando ao desenvolvimento de um teste sorológico baseado no ELISA, são o próximo passo para produzir um teste de diagnóstico confiável, acessível e útil para o diagnóstico da EMC no Brasil. |
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| AbstractList | Abstract Ehrlichia canis is the main etiological agent of canine monocytic ehrlichiosis (CME), a globally canine infectious disease. In Brazil, CME is considered to be endemic, and its prevalence can reach 65% in some states. The diagnosis of ehrlichiosis is important for treatment and epidemiological purposes. The E. canis TRP36 (Tandem Repeat Protein) protein elicits the earliest acute-phase antibody response observed during the course of the disease. This study aimed to generate the recombinant TRP36 protein from E. canis São Paulo strain and to evaluate its potential as a tool for the serologic diagnosis of CME. The E. canis São Paulo isolate was cultivated in DH82 lineage cells, and its genomic DNA was obtained. The bacterial DNA fragment encoding the entire ORF of TRP36 was cloned into the pBAD/Thio-TOPO vector and transformed into Escherichia coli DH10B competent cells with the trp36-bearing plasmid for protein expression. To evaluate the protein antigenicity, 16 canine serum samples were previously tested (by PCR and the commercial SNAP®4Dx® serological test). The results were in accordance with the SNAP®4Dx® test. Experiments using this recombinant protein as an antigen, targeting the development of a serologic test based on ELISA methodology, are the next step to produce a reliable, affordable and useful diagnostic tool for CME in Brazil.
Resumo Ehrlichia canis é o principal agente etiológico da erliquiose monocítica canina (EMC), uma doença infecciosa canina globalmente dispersa. No Brasil, a EMC é considerada endêmica, e a infecção pode atingir 65% em cães em alguns estados. O diagnóstico de erliquiose é importante para fins de tratamento e epidemiológicos. A proteína TRP36 de E. canis leva a uma resposta humoral com produção de anticorpos em fase aguda, encontrada durante o curso da doença. O objetivo deste estudo foi obter a proteína TRP36 recombinante da amostra São Paulo de E. canis e avaliar seu potencial como ferramenta para o diagnóstico sorológico da CME. O isolado de E. canis São Paulo foi cultivado em células da linhagem DH82 e o DNA genômico foi obtido. O fragmento de DNA bacteriano que codifica toda a ORF de TRP36 foi clonado no vetor pBAD / Thio-TOPO e transformado em células competentes Escherichia coli DH10B, com o plasmídeo portador de trp36 para expressão de proteínas. Para avaliar a antigenicidade da proteína, 16 amostras de soro canino foram previamente analisadas (por PCR e teste sorológico comercial SNAP®4Dx®). Os resultados estavam de acordo com o teste SNAP®4Dx®. Os experimentos que utilizam essa proteína recombinante como antígeno, visando ao desenvolvimento de um teste sorológico baseado no ELISA, são o próximo passo para produzir um teste de diagnóstico confiável, acessível e útil para o diagnóstico da EMC no Brasil. Abstract Ehrlichia canis is the main etiological agent of canine monocytic ehrlichiosis (CME), a globally canine infectious disease. In Brazil, CME is considered to be endemic, and its prevalence can reach 65% in some states. The diagnosis of ehrlichiosis is important for treatment and epidemiological purposes. The E. canis TRP36 (Tandem Repeat Protein) protein elicits the earliest acute-phase antibody response observed during the course of the disease. This study aimed to generate the recombinant TRP36 protein from E. canis São Paulo strain and to evaluate its potential as a tool for the serologic diagnosis of CME. The E. canis São Paulo isolate was cultivated in DH82 lineage cells, and its genomic DNA was obtained. The bacterial DNA fragment encoding the entire ORF of TRP36 was cloned into the pBAD/Thio-TOPO vector and transformed into Escherichia coli DH10B competent cells with the trp36-bearing plasmid for protein expression. To evaluate the protein antigenicity, 16 canine serum samples were previously tested (by PCR and the commercial SNAP®4Dx® serological test). The results were in accordance with the SNAP®4Dx® test. Experiments using this recombinant protein as an antigen, targeting the development of a serologic test based on ELISA methodology, are the next step to produce a reliable, affordable and useful diagnostic tool for CME in Brazil. |
| Author | Labruna, Marcelo Bahia Ferreira, Eliane de Oliveira Machado, Sérgio Lisboa Soares, João Fábio Toma, Helena Keiko Almosny, Nádia Regina Pereira Medeiros, Miguel Ângelo da Silva Matta, Maria Adelaide do Valle Xavier, Márcia de Souza Meirelles, Maria de Nazareth Silveira Leal de Silva, Maria Helena da |
| AuthorAffiliation | Universidade Castelo Branco Universidade Federal Fluminense Fundação Oswaldo Cruz Universidade de São Paulo Universidade Federal do Rio de Janeiro Universidade Federal do Rio Grande do Sul |
| AuthorAffiliation_xml | – name: Fundação Oswaldo Cruz – name: Universidade Federal do Rio Grande do Sul – name: Universidade de São Paulo – name: Universidade Federal do Rio de Janeiro – name: Universidade Castelo Branco – name: Universidade Federal Fluminense |
| Author_xml | – sequence: 1 givenname: Miguel Ângelo da Silva surname: Medeiros fullname: Medeiros, Miguel Ângelo da Silva organization: Universidade Castelo Branco, Brasil; Universidade Federal Fluminense, Brasil – sequence: 2 givenname: Maria Helena da surname: Silva fullname: Silva, Maria Helena da organization: Universidade Federal do Rio de Janeiro, Brasil – sequence: 3 givenname: Maria Adelaide do Valle surname: Matta fullname: Matta, Maria Adelaide do Valle organization: Fundação Oswaldo Cruz, Brasil – sequence: 4 givenname: Eliane de Oliveira orcidid: 0000-0002-9977-6460 surname: Ferreira fullname: Ferreira, Eliane de Oliveira organization: Universidade Federal do Rio de Janeiro, Brasil – sequence: 5 givenname: Sérgio Lisboa surname: Machado fullname: Machado, Sérgio Lisboa organization: Universidade Federal do Rio de Janeiro, Brasil – sequence: 6 givenname: João Fábio surname: Soares fullname: Soares, João Fábio organization: Universidade Federal do Rio Grande do Sul, Brasil – sequence: 7 givenname: Marcelo Bahia surname: Labruna fullname: Labruna, Marcelo Bahia organization: Universidade de São Paulo, Brasil – sequence: 8 givenname: Helena Keiko surname: Toma fullname: Toma, Helena Keiko organization: Universidade Federal do Rio de Janeiro, Brasil – sequence: 9 givenname: Márcia de Souza surname: Xavier fullname: Xavier, Márcia de Souza organization: Universidade Federal Fluminense, Brasil – sequence: 10 givenname: Maria de Nazareth Silveira Leal de surname: Meirelles fullname: Meirelles, Maria de Nazareth Silveira Leal de organization: Universidade Castelo Branco, Brasil; Fundação Oswaldo Cruz, Brasil – sequence: 11 givenname: Nádia Regina Pereira surname: Almosny fullname: Almosny, Nádia Regina Pereira organization: Universidade Federal Fluminense, Brasil |
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| Cites_doi | 10.1590/S1984-29612011000100002 10.1016/j.tvjl.2008.07.013 10.1016/j.vetmic.2013.02.015 10.1128/CDLI.6.3.392-399.1999 10.1038/227680a0 10.1093/jmedent/41.5.126 10.1128/CVI.00361-06 10.4142/jvs.2014.15.2.241 10.1016/S0195-5616(03)00031-7 10.1128/IAI.01494-06 10.1007/BF02623551 10.1128/JCM.29.9.2024-2029.1991 10.1590/S1517-83822008000300014 10.1093/nar/7.6.1513 10.1128/JCM.32.9.2107-2112.1994 10.1016/j.ttbdis.2015.10.003 10.1016/j.ttbdis.2014.01.011 10.1016/j.tvjl.2010.02.001 10.1177/104063879600800406 10.1128/IAI.71.5.2516-2524.2003 10.1128/IAI.74.1.711-720.2006 10.1590/S1984-29612014055 10.2460/ajvr.67.2.206 10.1196/annals.1355.079 10.1128/JCM.39.1.315-322.2001 10.1128/IAI.01347-10 10.1128/JCM.36.9.2671-2680.1998 |
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| DocumentTitleAlternate | Expressão e análise antigênica da proteína RTP36 recombinante da amostra São Paulo de Ehrlichia canis para testes sorológicos |
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| Keywords | TRP36 protein epidemiology diagnóstico diagnostic proteína TRP36 epidemiologia Ehrlichia canis serologic tests testes sorológicos |
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| References | McBride JW (ref21) 1996; 8 Donatien A (ref11) 1935; 28 Aguiar DM (ref2) 2008; 39 Birnboim HC (ref8) 1979; 7 Ferreira RF (ref14) 2014; 23 McBride JW (ref23) 1999; 6 Aguiar DM (ref4) 2016; 7 Groves MG (ref15) 1975; 36 Alves RN (ref7) 2014; 15 Laemmli UK (ref18) 1970; 227 Rikihisa Y (ref29) 1991; 29 O’Connor TP (ref26) 2006; 67 Cárdenas AM (ref9) 2007; 14 Aguirre E (ref6) 2009; 182 Doyle CK (ref12) 2005; 1063 Kuehn NF (ref17) 1985; 186 McBride JW (ref22) 2007; 75 Nyindo MB (ref25) 1971; 32 Zweygarth E (ref35) 2014; 5 McBride JW (ref24) 2011; 79 Cohn LA (ref10) 2003; 33 Ristic M (ref30) 1993 Wellman ML (ref34) 1988; 24 McBride JW (ref20) 2003; 71 Harrus S (ref16) 2011; 187 Aguiar DM (ref3) 2007; 37 Torres HM (ref32) 2002; 9 Ohashi N (ref27) 1998; 36 McBride JW (ref19) 2001; 39 Doyle CK (ref13) 2006; 74 Aguiar DM (ref1) 2007; 44 Rikihisa Y (ref28) 1994; 32 Aguiar DM (ref5) 2013; 164 Vieira RFC (ref33) 2011; 20 Rozen S (ref31) 2000 Cohn, LA 2003; 33 Vieira, RFC; Biondo, AW; Guimarães, AM; Santos, AP; Santos, RP; Dutra, LH 2011; 20 Rikihisa, Y; Ewing, SA; Fox, JC 1994; 32 O’Connor, TP; Hanscom, JL; Hegarty, BC; Groat, RG; Breitschwerdt, EB 2006; 67 Zweygarth, E; Cabezas-Cruz, A; Josemans, AI; Oosthuizen, MC; Matjila, PT; Lis, K 2014; 5 Ohashi, N; Unver, A; Zhi, N; Rikihisa, Y 1998; 36 Harrus, S; Waner, T 2011; 187 Nyindo, MB; Ristic, M; Huxsoll, DL; Smith, AR 1971; 32 Ristic, M; Holland, CJ; Woldehiwet, Z; Ristic, M 1993 Doyle, CK; Cardénas, AM; Aguiar, DM; Labruna, MB; Ndip, LM; Yu, XJ 2005; 1063 Aguiar, DM; Saito, TB; Hagiwara, MK; Machado, RZ; Labruna, MB 2007; 37 Aguirre, E; Ayllón, T; Sainz, A; Amusategui, I; Villaescusa, A; Rodríguez-Franco, F 2009; 182 Groves, MG; Dennis, GL; Amyx, HL; Huxsoll, DL 1975; 36 McBride, JW; Corstvet, RE; Breitschwerdt, EB; Walker, DH 2001; 39 Torres, HM; Massard, CL; Figueiredo, MJ; Ferreira, T; Almosny, NRP 2002; 9 Rikihisa, Y 1991; 29 Aguiar, DM; Cavalcante, GT; Pinter, A; Gennari, SM; Camargo, LMA; Labruna, MB 2007; 44 Alves, RN; Rieck, SE; Ueira-Vieira, C; Labruna, MB; Beletti, ME 2014; 15 McBride, JW; Corstvet, RE; Gaunt, SD; Boudreaux, C; Guedry, T; Walker, DH 2003; 71 Doyle, CK; Nethery, KA; Popov, VL; McBride, JW 2006; 74 McBride, JW; Doyle, CK; Zhang, X; Cardénas, AM; Popov, VL; Nethery, KA 2007; 75 Ferreira, RF; Cerqueira, AMF; Castro, TX; Ferreira, EO; Neves, FPG; Barbosa, AV 2014; 23 Cárdenas, AM; Doyle, CK; Zhang, X; Nethery, K; Corstvet, RE; Walker, DH 2007; 14 Laemmli, UK 1970; 227 Aguiar, DM; Zhang, X; Melo, AL; Pacheco, TA; Meneses, AM; Zanutto, MS 2013; 164 Birnboim, HC; Doly, J 1979; 7 Wellman, ML; Krakowka, S; Jacobs, RM; Kociba, GJ 1988; 24 Donatien, A; Lestoquard, F 1935; 28 McBride, JW; Zhang, X; Wakeel, A; Kuriakose, JA 2011; 79 McBride, JW; Yu, XJ; Walker, DH 1999; 6 Rozen, S; Skaletsky, HJ; Misener, S; Krawetz, S 2000 Kuehn, NF; Gaunt, SD 1985; 186 Aguiar, DM; Zhang, X; Braga, IA; Taques, II; McBride, JW 2016; 7 Aguiar, DM; Hagiwara, MK; Labruna, MB 2008; 39 McBride, JW; Corstvet, RE; Gaunt, SD; Chinsangaram, J; Akita, GY; Osburn, BI 1996; 8 |
| References_xml | – volume: 20 start-page: 1 issue: 1 year: 2011 ident: ref33 article-title: Ehrlichiosis in Brazil publication-title: Rev Bras Parasitol Vet doi: 10.1590/S1984-29612011000100002 contributor: fullname: Vieira RFC – volume: 182 start-page: 301 issue: 2 year: 2009 ident: ref6 article-title: Results from an indirect fluorescent antibody test using three different strains of Ehrlichia canis. publication-title: Vet J doi: 10.1016/j.tvjl.2008.07.013 contributor: fullname: Aguirre E – volume: 164 start-page: 315 issue: 3-4 year: 2013 ident: ref5 article-title: Genetic diversity of Ehrlichia canis in Brazil publication-title: Vet Microbiol doi: 10.1016/j.vetmic.2013.02.015 contributor: fullname: Aguiar DM – volume: 6 start-page: 392 issue: 3 year: 1999 ident: ref23 article-title: Molecular cloning of the gene for a conserved major immunoreactive 28-kilodalton protein of Ehrlichia canis: a potential serodiagnostic antigen publication-title: Clin Diagn Lab Immunol doi: 10.1128/CDLI.6.3.392-399.1999 contributor: fullname: McBride JW – volume: 37 start-page: 796 issue: 3 year: 2007 ident: ref3 article-title: Serological diagnosis of canine monocytic ehrlichiosis with Brazilian antigen of Ehrlichia canis. publication-title: Cienc Rural contributor: fullname: Aguiar DM – volume: 227 start-page: 680 issue: 5259 year: 1970 ident: ref18 article-title: Cleavage of structural proteins during the assembly of the head of bacteriophage T4 publication-title: Nature doi: 10.1038/227680a0 contributor: fullname: Laemmli UK – volume: 32 start-page: 1651 issue: 11 year: 1971 ident: ref25 article-title: Tropical canine pancytopenia: in vitro cultivation of the causative agent- Ehrlichia canis. publication-title: Am J Vet Res contributor: fullname: Nyindo MB – volume: 44 start-page: 126 issue: 1 year: 2007 ident: ref1 article-title: Prevalence of Ehrlichia canis (Rickettsiales: Anaplasmataceae) in dogs and Rhipicephalus sanguineus (Acari: Ixodidae) ticks from Brazil publication-title: J Med Entomol doi: 10.1093/jmedent/41.5.126 contributor: fullname: Aguiar DM – volume: 14 start-page: 123 issue: 2 year: 2007 ident: ref9 article-title: Enzyme-linked immunosorbent assay with conserved immunoreactive glycoproteins gp36 and gp19 has enhanced sensitivity and provides species-specific immunodiagnosis of Ehrlichia canis infection publication-title: Clin Vaccine Immunol doi: 10.1128/CVI.00361-06 contributor: fullname: Cárdenas AM – volume: 15 start-page: 241 issue: 2 year: 2014 ident: ref7 article-title: Isolation, in vitro propagation, genetic analysis, and immunogenic characterization of an Ehrlichia canis strain from southeastern Brazil publication-title: J Vet Sci doi: 10.4142/jvs.2014.15.2.241 contributor: fullname: Alves RN – volume: 33 start-page: 863 issue: 4 year: 2003 ident: ref10 article-title: Ehrlichiosis and related infections publication-title: Vet Clin North Am Small Anim Pract doi: 10.1016/S0195-5616(03)00031-7 contributor: fullname: Cohn LA – volume: 28 start-page: 418 year: 1935 ident: ref11 article-title: Existence en Algérie d’une Rickettsia du chien publication-title: Bull Soc Pathol Exot contributor: fullname: Donatien A – volume: 75 start-page: 74 issue: 1 year: 2007 ident: ref22 article-title: Identification of a glycosylated Ehrlichia canis 19-Kilodalton major immunoreactive protein with a species-specific serine-rich glycopeptide epitope publication-title: Infect Immun doi: 10.1128/IAI.01494-06 contributor: fullname: McBride JW – volume: 24 start-page: 223 issue: 3 year: 1988 ident: ref34 article-title: A macrophage-monocyte cell line from a dog with malignant histiocytosis publication-title: In Vitro Cell Dev Biol doi: 10.1007/BF02623551 contributor: fullname: Wellman ML – volume: 29 start-page: 2024 issue: 9 year: 1991 ident: ref29 article-title: Cross-Reacting Antigens between Neorickettsia helminthoeca and Ehrlichia Species, shown by Immunofluorescence and Western Immunoblotting publication-title: J Clin Microbiol doi: 10.1128/JCM.29.9.2024-2029.1991 contributor: fullname: Rikihisa Y – volume: 36 start-page: 937 issue: 7 year: 1975 ident: ref15 article-title: Transmission of Ehrlichia canis to dogs by ticks (Rhipicephalus sanguineus) publication-title: Am J Vet Res contributor: fullname: Groves MG – volume: 9 start-page: 77 issue: 2 year: 2002 ident: ref32 article-title: Isolamento e propagação da Ehrlichia canis em células DH82 e obtenção de antígeno para a reação de imunofluorescência indireta publication-title: Rev Bras Cienc Vet contributor: fullname: Torres HM – volume: 39 start-page: 489 issue: 3 year: 2008 ident: ref2 article-title: In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil publication-title: Braz J Microbiol doi: 10.1590/S1517-83822008000300014 contributor: fullname: Aguiar DM – volume: 7 start-page: 1513 issue: 6 year: 1979 ident: ref8 article-title: A rapid alkaline extraction procedure for screening recombinant plasmid DNA publication-title: Nucleic Acids Res doi: 10.1093/nar/7.6.1513 contributor: fullname: Birnboim HC – volume: 32 start-page: 2107 issue: 9 year: 1994 ident: ref28 article-title: Western immunoblot analysis of Ehrlichia chaffeensis, E. canis, or E. ewingii infections in dogs and humans publication-title: J Clin Microbiol doi: 10.1128/JCM.32.9.2107-2112.1994 contributor: fullname: Rikihisa Y – start-page: 365 volume-title: Primer3 on the WWW for general users and for biologist programmers. year: 2000 ident: ref31 contributor: fullname: Rozen S – volume: 7 start-page: 142 issue: 1 year: 2016 ident: ref4 article-title: Detection of genotype-specific Ehrlichia canis exposure in Brazilian dogs by TRP36 peptide ELISA publication-title: Ticks Tick Borne Dis doi: 10.1016/j.ttbdis.2015.10.003 contributor: fullname: Aguiar DM – volume: 5 start-page: 423 issue: 4 year: 2014 ident: ref35 article-title: In vitro culture and structural differences in the major immunoreactive protein gp36 of geographically distant Ehrlichia canis isolates publication-title: Ticks Tick Borne Dis doi: 10.1016/j.ttbdis.2014.01.011 contributor: fullname: Zweygarth E – volume: 187 start-page: 292 issue: 3 year: 2011 ident: ref16 article-title: Diagnosis of canine monocytotropic ehrlichiosis (Ehrlichia canis): an overview publication-title: Vet J doi: 10.1016/j.tvjl.2010.02.001 contributor: fullname: Harrus S – volume: 8 start-page: 441 issue: 4 year: 1996 ident: ref21 article-title: PCR detection of acute Ehrlichia canis infection in dogs publication-title: J Vet Diagn Invest doi: 10.1177/104063879600800406 contributor: fullname: McBride JW – volume: 71 start-page: 2516 issue: 5 year: 2003 ident: ref20 article-title: Kinetics of Antibody Response to Ehrlichia canis immunoreactive proteins publication-title: Infect Immun doi: 10.1128/IAI.71.5.2516-2524.2003 contributor: fullname: McBride JW – volume: 74 start-page: 711 issue: 1 year: 2006 ident: ref13 article-title: Differentially expressed and secreted major immunoreactive protein orthologs of Ehrlichia canis and E. chaffeensis elicit early antibody responses to epitopes on glycosylated tandem repeats publication-title: Infect Immun doi: 10.1128/IAI.74.1.711-720.2006 contributor: fullname: Doyle CK – volume: 23 start-page: 301 issue: 3 year: 2014 ident: ref14 article-title: Genetic diversity of Ehrlichia canis strains from naturally infected dogs in Rio de Janeiro, Brazil publication-title: Rev Bras Parasitol Vet doi: 10.1590/S1984-29612014055 contributor: fullname: Ferreira RF – volume: 186 start-page: 355 issue: 4 year: 1985 ident: ref17 article-title: Clinical and hematologic findings in canine ehrlichiosis publication-title: J Am Vet Med Assoc contributor: fullname: Kuehn NF – volume: 67 start-page: 206 issue: 2 year: 2006 ident: ref26 article-title: Comparison of an indirect immunofluorescence assay, western blot analysis, and a commercially available ELISA for detection of Ehrlichia canis antibodies in canine sera publication-title: Am J Vet Res doi: 10.2460/ajvr.67.2.206 contributor: fullname: O’Connor TP – volume: 1063 start-page: 433 issue: 1 year: 2005 ident: ref12 article-title: Molecular characterization of E. canis gp36 and E. chaffeensis gp47 tandem repeats among isolates from different geographic localization publication-title: Ann N Y Acad Sci doi: 10.1196/annals.1355.079 contributor: fullname: Doyle CK – volume: 39 start-page: 315 issue: 1 year: 2001 ident: ref19 article-title: Immunodiagnosis of Ehrlichia canis infection with recombinant proteins publication-title: J Clin Microbiol doi: 10.1128/JCM.39.1.315-322.2001 contributor: fullname: McBride JW – volume: 79 start-page: 3178 issue: 8 year: 2011 ident: ref24 article-title: Tyrosine-phosphorylated Ehrlichia chaffeensis and Ehrlichia canis tandem repeat orthologs contain a major continuous cross-reactive antibody epitope in lysine-rich repeats publication-title: Infect Immun doi: 10.1128/IAI.01347-10 contributor: fullname: McBride JW – start-page: 169 volume-title: Canine Ehrlichiosis. year: 1993 ident: ref30 contributor: fullname: Ristic M – volume: 36 start-page: 2671 issue: 9 year: 1998 ident: ref27 article-title: Cloning and characterization of multigenes encoding the immunodominant 30-kilodalton major outer membrane proteins of Ehrlichia canis and application of the recombinant protein for serodiagnosis publication-title: J Clin Microbiol doi: 10.1128/JCM.36.9.2671-2680.1998 contributor: fullname: Ohashi N – volume: 6 start-page: 392 issue: 3 year: 1999 end-page: 399 article-title: Molecular cloning of the gene for a conserved major immunoreactive 28-kilodalton protein of Ehrlichia canis: a potential serodiagnostic antigen publication-title: Clin Diagn Lab Immunol contributor: fullname: McBride, JW; Yu, XJ; Walker, DH – volume: 32 start-page: 1651 issue: 11 year: 1971 end-page: 1658 article-title: Tropical canine pancytopenia: in vitro cultivation of the causative agent- Ehrlichia canis. publication-title: Am J Vet Res contributor: fullname: Nyindo, MB; Ristic, M; Huxsoll, DL; Smith, AR – volume: 75 start-page: 74 issue: 1 year: 2007 end-page: 82 article-title: Identification of a glycosylated Ehrlichia canis 19-Kilodalton major immunoreactive protein with a species-specific serine-rich glycopeptide epitope publication-title: Infect Immun contributor: fullname: McBride, JW; Doyle, CK; Zhang, X; Cardénas, AM; Popov, VL; Nethery, KA – start-page: 169 year: 1993 end-page: 186 publication-title: Rickettsial and Chlamydial Diseases of Domestic Animals contributor: fullname: Ristic, M; Holland, CJ; Woldehiwet, Z; Ristic, M – volume: 1063 start-page: 433 issue: 1 year: 2005 end-page: 435 article-title: Molecular characterization of E. canis gp36 and E. chaffeensis gp47 tandem repeats among isolates from different geographic localization publication-title: Ann N Y Acad Sci contributor: fullname: Doyle, CK; Cardénas, AM; Aguiar, DM; Labruna, MB; Ndip, LM; Yu, XJ – volume: 74 start-page: 711 issue: 1 year: 2006 end-page: 720 article-title: Differentially expressed and secreted major immunoreactive protein orthologs of Ehrlichia canis and E. chaffeensis elicit early antibody responses to epitopes on glycosylated tandem repeats publication-title: Infect Immun contributor: fullname: Doyle, CK; Nethery, KA; Popov, VL; McBride, JW – volume: 71 start-page: 2516 issue: 5 year: 2003 end-page: 2524 article-title: Kinetics of Antibody Response to Ehrlichia canis immunoreactive proteins publication-title: Infect Immun contributor: fullname: McBride, JW; Corstvet, RE; Gaunt, SD; Boudreaux, C; Guedry, T; Walker, DH – volume: 8 start-page: 441 issue: 4 year: 1996 end-page: 447 article-title: PCR detection of acute Ehrlichia canis infection in dogs publication-title: J Vet Diagn Invest contributor: fullname: McBride, JW; Corstvet, RE; Gaunt, SD; Chinsangaram, J; Akita, GY; Osburn, BI – volume: 39 start-page: 489 issue: 3 year: 2008 end-page: 493 article-title: In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil publication-title: Braz J Microbiol contributor: fullname: Aguiar, DM; Hagiwara, MK; Labruna, MB – volume: 29 start-page: 2024 issue: 9 year: 1991 end-page: 2029 article-title: Cross-Reacting Antigens between Neorickettsia helminthoeca and Ehrlichia Species, shown by Immunofluorescence and Western Immunoblotting publication-title: J Clin Microbiol contributor: fullname: Rikihisa, Y – volume: 9 start-page: 77 issue: 2 year: 2002 end-page: 82 article-title: Isolamento e propagação da Ehrlichia canis em células DH82 e obtenção de antígeno para a reação de imunofluorescência indireta publication-title: Rev Bras Cienc Vet contributor: fullname: Torres, HM; Massard, CL; Figueiredo, MJ; Ferreira, T; Almosny, NRP – volume: 36 start-page: 2671 issue: 9 year: 1998 end-page: 2680 article-title: Cloning and characterization of multigenes encoding the immunodominant 30-kilodalton major outer membrane proteins of Ehrlichia canis and application of the recombinant protein for serodiagnosis publication-title: J Clin Microbiol contributor: fullname: Ohashi, N; Unver, A; Zhi, N; Rikihisa, Y – volume: 7 start-page: 1513 issue: 6 year: 1979 end-page: 1523 article-title: A rapid alkaline extraction procedure for screening recombinant plasmid DNA publication-title: Nucleic Acids Res contributor: fullname: Birnboim, HC; Doly, J – volume: 23 start-page: 301 issue: 3 year: 2014 end-page: 308 article-title: Genetic diversity of Ehrlichia canis strains from naturally infected dogs in Rio de Janeiro, Brazil publication-title: Rev Bras Parasitol Vet contributor: fullname: Ferreira, RF; Cerqueira, AMF; Castro, TX; Ferreira, EO; Neves, FPG; Barbosa, AV – volume: 187 start-page: 292 issue: 3 year: 2011 end-page: 296 article-title: Diagnosis of canine monocytotropic ehrlichiosis (Ehrlichia canis): an overview publication-title: Vet J contributor: fullname: Harrus, S; Waner, T – volume: 227 start-page: 680 issue: 5259 year: 1970 end-page: 685 article-title: Cleavage of structural proteins during the assembly of the head of bacteriophage T4 publication-title: Nature contributor: fullname: Laemmli, UK – volume: 15 start-page: 241 issue: 2 year: 2014 end-page: 248 article-title: Isolation, in vitro propagation, genetic analysis, and immunogenic characterization of an Ehrlichia canis strain from southeastern Brazil publication-title: J Vet Sci contributor: fullname: Alves, RN; Rieck, SE; Ueira-Vieira, C; Labruna, MB; Beletti, ME – volume: 14 start-page: 123 issue: 2 year: 2007 end-page: 128 article-title: Enzyme-linked immunosorbent assay with conserved immunoreactive glycoproteins gp36 and gp19 has enhanced sensitivity and provides species-specific immunodiagnosis of Ehrlichia canis infection publication-title: Clin Vaccine Immunol contributor: fullname: Cárdenas, AM; Doyle, CK; Zhang, X; Nethery, K; Corstvet, RE; Walker, DH – volume: 186 start-page: 355 issue: 4 year: 1985 end-page: 358 article-title: Clinical and hematologic findings in canine ehrlichiosis publication-title: J Am Vet Med Assoc contributor: fullname: Kuehn, NF; Gaunt, SD – volume: 182 start-page: 301 issue: 2 year: 2009 end-page: 305 article-title: Results from an indirect fluorescent antibody test using three different strains of Ehrlichia canis. publication-title: Vet J contributor: fullname: Aguirre, E; Ayllón, T; Sainz, A; Amusategui, I; Villaescusa, A; Rodríguez-Franco, F – volume: 79 start-page: 3178 issue: 8 year: 2011 end-page: 3187 article-title: Tyrosine-phosphorylated Ehrlichia chaffeensis and Ehrlichia canis tandem repeat orthologs contain a major continuous cross-reactive antibody epitope in lysine-rich repeats publication-title: Infect Immun contributor: fullname: McBride, JW; Zhang, X; Wakeel, A; Kuriakose, JA – volume: 20 start-page: 1 issue: 1 year: 2011 end-page: 12 article-title: Ehrlichiosis in Brazil publication-title: Rev Bras Parasitol Vet contributor: fullname: Vieira, RFC; Biondo, AW; Guimarães, AM; Santos, AP; Santos, RP; Dutra, LH – volume: 32 start-page: 2107 issue: 9 year: 1994 end-page: 2112 article-title: Western immunoblot analysis of Ehrlichia chaffeensis, E. canis, or E. ewingii infections in dogs and humans publication-title: J Clin Microbiol contributor: fullname: Rikihisa, Y; Ewing, SA; Fox, JC – volume: 7 start-page: 142 issue: 1 year: 2016 end-page: 145 article-title: Detection of genotype-specific Ehrlichia canis exposure in Brazilian dogs by TRP36 peptide ELISA publication-title: Ticks Tick Borne Dis contributor: fullname: Aguiar, DM; Zhang, X; Braga, IA; Taques, II; McBride, JW – volume: 67 start-page: 206 issue: 2 year: 2006 end-page: 210 article-title: Comparison of an indirect immunofluorescence assay, western blot analysis, and a commercially available ELISA for detection of Ehrlichia canis antibodies in canine sera publication-title: Am J Vet Res contributor: fullname: O’Connor, TP; Hanscom, JL; Hegarty, BC; Groat, RG; Breitschwerdt, EB – volume: 28 start-page: 418 year: 1935 end-page: 419 article-title: Existence en Algérie d’une Rickettsia du chien publication-title: Bull Soc Pathol Exot contributor: fullname: Donatien, A; Lestoquard, F – volume: 164 start-page: 315 issue: 3-4 year: 2013 end-page: 321 article-title: Genetic diversity of Ehrlichia canis in Brazil publication-title: Vet Microbiol contributor: fullname: Aguiar, DM; Zhang, X; Melo, AL; Pacheco, TA; Meneses, AM; Zanutto, MS – volume: 44 start-page: 126 issue: 1 year: 2007 end-page: 132 article-title: Prevalence of Ehrlichia canis (Rickettsiales: Anaplasmataceae) in dogs and Rhipicephalus sanguineus (Acari: Ixodidae) ticks from Brazil publication-title: J Med Entomol contributor: fullname: Aguiar, DM; Cavalcante, GT; Pinter, A; Gennari, SM; Camargo, LMA; Labruna, MB – volume: 33 start-page: 863 issue: 4 year: 2003 end-page: 884 article-title: Ehrlichiosis and related infections publication-title: Vet Clin North Am Small Anim Pract contributor: fullname: Cohn, LA – volume: 24 start-page: 223 issue: 3 year: 1988 end-page: 229 article-title: A macrophage-monocyte cell line from a dog with malignant histiocytosis publication-title: In Vitro Cell Dev Biol contributor: fullname: Wellman, ML; Krakowka, S; Jacobs, RM; Kociba, GJ – volume: 37 start-page: 796 issue: 3 year: 2007 end-page: 802 article-title: Serological diagnosis of canine monocytic ehrlichiosis with Brazilian antigen of Ehrlichia canis. publication-title: Cienc Rural contributor: fullname: Aguiar, DM; Saito, TB; Hagiwara, MK; Machado, RZ; Labruna, MB – volume: 39 start-page: 315 issue: 1 year: 2001 end-page: 322 article-title: Immunodiagnosis of Ehrlichia canis infection with recombinant proteins publication-title: J Clin Microbiol contributor: fullname: McBride, JW; Corstvet, RE; Breitschwerdt, EB; Walker, DH – start-page: 365 year: 2000 end-page: 386 publication-title: Bioinformatics Methods and Protocols: Methods in Molecular Biology contributor: fullname: Rozen, S; Skaletsky, HJ; Misener, S; Krawetz, S – volume: 36 start-page: 937 issue: 7 year: 1975 end-page: 940 article-title: Transmission of Ehrlichia canis to dogs by ticks (Rhipicephalus sanguineus) publication-title: Am J Vet Res contributor: fullname: Groves, MG; Dennis, GL; Amyx, HL; Huxsoll, DL – volume: 5 start-page: 423 issue: 4 year: 2014 end-page: 431 article-title: In vitro culture and structural differences in the major immunoreactive protein gp36 of geographically distant Ehrlichia canis isolates publication-title: Ticks Tick Borne Dis contributor: fullname: Zweygarth, E; Cabezas-Cruz, A; Josemans, AI; Oosthuizen, MC; Matjila, PT; Lis, K |
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| SubjectTerms | diagnostic Ehrlichia canis epidemiology PARASITOLOGY serologic tests TRP36 protein VETERINARY SCIENCES |
| Title | Expression and antigenic analysis of the recombinant TRP36 protein from Ehrlichia canis São Paulo strain for serologic tests |
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